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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of Hormonal Supplementation on Nuclear Maturation and Cortical Granules Distribution of Canine Oocytes During Various Reproductive Stages

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Author(s):
Apparicio, M. [1] ; Alves, A. E. [1] ; Pires-Butler, E. A. [1] ; Ribeiro, A. P. C. [2] ; Covizzi, G. J. [3] ; Vicente, W. R. R. [1]
Total Authors: 6
Affiliation:
[1] UNESP Univ Estadual Paulista, Fac Agr & Vet Sci, Dept Prevent Med & Anim Reprod, Jaboticabal, SP - Brazil
[2] Univ Fed Tocantins, Escola Med Vet & Zootecnia, Araguaina, TO - Brazil
[3] UNIME Uniao Metropolitana Desenvolvimento Ensino, Lauro De Freitas, BA - Brazil
Total Affiliations: 3
Document type: Journal article
Source: REPRODUCTION IN DOMESTIC ANIMALS; v. 46, n. 5, p. 896-903, OCT 2011.
Web of Science Citations: 4
Abstract

The aim of this study was to evaluate the effects of hCG, progesterone and oestradiol supplementation on nuclear and cytoplasmic maturation of canine oocytes cultured for 24, 48, 72 and 96 h. Oocytes obtained from 18 healthy bitches were divided into three groups according to their reproductive status (follicular, luteal and anoestrus stages) and cultured in TCM 199 + 25 UI/ml of hCG + 1 mu g/ml of progesterone + 1 mu g/ml of 17-beta oestradiol or without hormonal supplementation (control) for different periods. Then, they were stained with FITC-LCA-Hoescht for chromatin configuration and cortical granules distribution and evaluated under an epifluorescence microscope. Culture time and the influence of different stages of the oestrous cycle were also evaluated. The present study demonstrated that there was no significant difference among the reproductive stages. With regards to culture medium, only oocytes from the supplemented medium were able to complete meiosis; however, significant difference was only noticed in the percentage of MI stage oocytes (p < 0.05) in the follicular and luteal group at 72 h of culture. Most oocytes in germinal vesicle, germinal vesicle breakdown and metaphase I stage had cortical granules distributed throughout the cytoplasm (immature pattern), irrespective of the culture period (p < 0.05). Cortical granules distributed immediately beneath the plasma membrane (mature) was only observed in metaphase II stage oocytes, but not all of them presented matured cytoplasm. Our results reveal that cortical granules distribution in canine oocytes matured in vitro did not progressed in correspondence with nuclear stage changes and are in accordance with those from other species. (AU)