| Full text | |
| Author(s): |
Total Authors: 3
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| Affiliation: | [1] Univ British Columbia, Forest Prod Biotechnol Bioenergy Grp, Vancouver, BC V5Z 1M9 - Canada
[2] Fdn Univ Fed Tocantis, Gurupi To - Brazil
[3] Univ Sao Paulo, Dept Biotechnol, Escola Engn Lorena, Lorena, SP - Brazil
Total Affiliations: 3
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| Document type: | Journal article |
| Source: | BIOMASS & BIOENERGY; v. 35, n. 9, p. 4040-4044, OCT 2011. |
| Web of Science Citations: | 6 |
| Abstract | |
Enzyme production is a growing field in biotechnology and increasing attention has been devoted to the solid-state fermentation (SSF) of lignocellulosic biomass for production of industrially relevant lignocellulose deconstruction enzymes, especially manganese-peroxidase (MnP), which plays a crucial role in lignin degradation. However, there is a scarcity of studies regarding extraction of the secreted metabolities that are commonly bound to the fermented solids, preventing their accurate detection and limiting recovery efficiency. In the present work, we assessed the effectiveness of extraction process variables (pH, stirring rate, temperature, and extraction time) on recovery efficiency of manganese-peroxidase (MnP) obtained by SSF of eucalyptus residues using Lentinula edodes using statistical design of experiments. The results from this study indicated that of the variables studied, pH was the most significant (p < 0.05%) parameter affecting MnP recovery yield, while temperature, extraction time, and stirring rate presented no statistically significant effects in the studied range. The optimum pH for extraction of MnP was at 4.0-5.0, which yielded 1500-1700 IU kg (1) of enzyme activity at extraction time 4-5 h, under static condition at room temperature. (C) 2011 Elsevier Ltd. All rights reserved. (AU) | |