Cobalt phthalocyanine as a biomimetic catalyst in the amperometric quantification of dipyrone using FIA

Author(s)

Boni, Ana Carolina ^[1] ; Wong, Ademar ^[1] ; Fireman Dutra, Rosa Amalia ^[2] ; Taboada Sotomayor, Maria Del Pilar ^[1]

Affiliation

^[1] State Univ Sao Paulo UNESP, Dept Analyt Chem, Inst Chem, BR-14801970 Araraquara, SP - Brazil

^[2] Univ Pernambuco, Lab Diagnost Res IAPED, BR-50100130 Recife, PE - Brazil

• Document type: Journal article
• Source: Talanta; v. 85, n. 4, p. 2067-2073, SEP 30 2011.
• Web of Science Citations: 22

Abstract

A biomimetic sensor for the determination of dipyrone was prepared by modifying carbon paste with cobalt phthalocyanine (CoPc), and used as an amperometric detector in a flow injection analysis (FIA) system. The results of cyclic voltammetry experiments suggested that CoPc behaved as a biomimetic catalyst in the electrocatalytic oxidation of dipyrone, which involved the transfer of one electron. The optimized FIA procedure employed a flow rate of 1.5 mL min(-1), a 75 mu L sample loop, a 0.1 mol L(-1) phosphate buffer carrier solution at pH 7.0 and amperometric detection at a potential of 0.3 V vs. Ag/AgCl. Under these conditions, the proposed method showed a linear response for dipyrone concentrations in the range 5.0 x 10(-6)-6.3 x 10(-3) mol L(-1). Selectivity and interference studies were carried out in order to validate the system for use with pharmaceutical and environmental samples. In addition to being environmentally friendly, the proposed method is a sensitive and selective analytical tool for the determination of dipyrone. (C) 2011 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 08/00303-5 - Construction of biomimetic sensors for drugs determination and their coupling in flow systems
• Grantee: Maria Del Pilar Taboada Sotomayor
• Support type: Regular Research Grants
• FAPESP's process: 09/04598-2 - Development of a flow analytical methodology for dypirone determination in drugs and effluents using biomimetic sensor as highly selective detectors
• Grantee: Ana Carolina Boni
• Support type: Scholarships in Brazil - Scientific Initiation