Exploring Strategic Approaches for Cv FAP Photodecarboxylation through Violet Light Irradiation

Author(s)

Breda, Gabriela C. ; Franca, Alexandre S. ; de Oliveira, Kleber T. ; Almeida, Rodrigo V. ; de Souza, Rodrigo O. M. A.

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. 35, n. 8, p. 5-pg., 2024-08-01.

Abstract

In this study, we describe a light -driven photocatalytic decarboxylation of palmitic acid and related fatty acids using Chlorella variabilis fatty acid photodecarboxylase ( Cv FAP). By utilizing violet light emitting diode (LED) light (50 W; 397 nm), we achieved a remarkable conversion efficiency of 99% within just 4 min, surpassing the previous 79% conversion achieved in 60 min using blue LED light (300 W; 439 nm). Importantly, the use of 50 W violet LED light also resulted in a lower enzyme photoinactivation rate when compared to 300 W blue LED. Comparing the whole -cell biocatalyst with the enzymatic extract, we found that the former demonstrated superior catalytic performance and reduced susceptibility to photoinactivation. Furthermore, whole -cell biocatalyst reuse was demonstrated after five sequential batches. Employing this approach, we successfully synthesized 26 mmol L -1 h -1 of pentadecane, showcasing a promising strategy to improve productivity. These findings represent a significant advancement in Cv FAP photodecarboxylation processes compared to the literature, utilizing an alternative light source, with potential implications to the biofuel sector. (AU)

• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 20/06874-6 - Applications of technologies and synthetic methodologies developed under continuous flow conditions at LQBO-UFSCar for the preparation of molecules with biological activities and APIs
• Grantee: Kleber Thiago de Oliveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/27176-8 - Asymmetric photocatalysis applied to the synthesis of Brivaracetam: synthetic approaches employing enabling technologies for chemical synthesis
• Grantee: Kleber Thiago de Oliveira
• Support Opportunities: Regular Research Grants