A functionally augmented carbohydrate utilization locus from herbivore gut microbiota fueled by dietary β-glucans

Author(s):Show less -

Mandelli, Fernanda ; Martins, Marcele Pandelo ; Chinaglia, Mariana ; de Lima, Evandro Antonio ; Morais, Mariana Abrahao Bueno ; Lima, Tatiani Brenelli ; Cabral, Lucelia ; Pirolla, Renan Augusto Siqueira ; Fuzita, Felipe Jun ; Paixao, Douglas Antonio Alvaredo ; Andrade, Maxuel de Oliveira ; Wolf, Lucia Daniela ; Vieira, Plinio Salmazo ; Persinoti, Gabriela Felix ; Murakami, Mario Tyago

• Document type: Journal article
• Source: NPJ BIOFILMS AND MICROBIOMES; v. 10, n. 1, p. 13-pg., 2024-10-14.

Abstract

Gut microbiota members from the Bacteroidota phylum play a pivotal role in mammalian health and metabolism. They thrive in this diverse ecosystem due to their notable ability to cope with distinct recalcitrant dietary glycans via polysaccharide utilization loci (PULs). Our study reveals that a PUL from an herbivore gut bacterium belonging to the Bacteroidota phylum, with a gene composition similar to that in the human gut, exhibits extended functionality. While the human gut PUL targets mixed-linkage beta-glucans specifically, the herbivore gut PUL also efficiently processes linear and substituted beta-1,3-glucans. This gain of function emerges from molecular adaptations in recognition proteins and carbohydrate-active enzymes, including a beta-glucosidase specialized for beta(1,6)-glucosyl linkages, a typical substitution in beta(1,3)-glucans. These findings broaden the existing model for non-cellulosic beta-glucans utilization by gut bacteria, revealing an additional layer of functional and evolutionary complexity within the gut microbiota, beyond conventional gene insertions/deletions to intricate biochemical interactions. (AU)

• FAPESP's process: 21/04891-3 - Enzymatic mechanisms from the microbiome of aquatic herbivores for depolymerization and metabolism of complex carbohydrates
• Grantee: Mário Tyago Murakami
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 22/03059-5 - Uncovering hidden microbial functions involved in adaptation and processing of synthetic pollutants
• Grantee: Gabriela Felix Persinoti
• Support Opportunities: Research Grants - Initial Project
• FAPESP's process: 21/09793-0 - Discovery and mechanistic investigation of carbohydrate-active enzymes from the gut microbiota of Manatee
• Grantee: Marcele Pandeló Martins
• Support Opportunities: Scholarships in Brazil - Post-Doctoral