(Na plus , K plus )- ATPase kinetics in Macrobrachium pantanalense: highlighting intra- and interspecific variation within the Macrobrachium amazonicum complex

Author(s)

Fabri, Leonardo M. ; Moraes, Cintya M. ; Calixto-Cunha, Marina ; Almeida, Ariadine C. ; Faleiros, Rogerio O. ; Garcon, Daniela P. ; McNamara, John C. ; Faria, Samuel C. ; Leone, Francisco A.

• Document type: Journal article
• Source: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY; v. 273, p. 11-pg., 2024-05-16.

Abstract

The Macrobrachium amazonicum complex is composed of at least the Macrobrachium amazonicum and Macrobrachium pantanalense species, with the latter described from specimens originally identified as part of an endemic M. amazonicum population in the Brazilian Pantanal region. While there may be a reproductive barrier between these two Macrobrachium species, both are phylogenetically close, with small genetic distance. However, there is currently no available biochemical information of Macrobrachium pantanalense (Na+, K+)-ATPase. Here, we report the kinetic characteristics of the gill (Na+, K+)-ATPase in two populations of M. pantanalense from Baiazinha Lagoon (Miranda, MS, Brazil) and Araguari River (Uberlandia, MG, Brazil), and compare them with Macrobrachium amazonicum populations from the Paran<acute accent>a-Paraguay River Basin. (Na+, K+)-ATPase activities were 67.9 +/- 3.4 and 93.3 +/- 4.1 nmol Pi min-1 mg-1 protein for the Baiazinha Lagoon and Araguari River populations, respectively. Two ATP hydrolyzing sites were observed for the Araguari River population while a single ATP site was observed for the Baiazinha Lagoon shrimps. Compared to the Araguari River population, a 3fold greater apparent affinity for Mg2+ and Na+ was estimated for the Baiazinha Lagoon population, but no difference in K+ affinity and ouabain inhibition was seen. The kinetic differences observed in the gill (Na+, K+)ATPase between the two populations of M. pantanalense, compared with those of various M. amazonicum populations, highlight interspecific divergence within the Macrobrachium genus, now examined from a biochemical perspective. (AU)

• FAPESP's process: 22/03105-7 - Using the phylogenetic past to predict climate impacts: ecophysiology of symbiosis and accelerated evolution to coral reef conservation
• Grantee: Samuel Coelho de Faria
• Support Opportunities: Research Grants - Initial Project
• FAPESP's process: 16/25336-0 - Comparative biochemical study of gill (Na+, K +)- ATPase of Macrobrachium amazonicum (Heller, 1862) inhabitant of coastal and continental regions
• Grantee: Francisco de Assis Leone
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/21899-8 - Kinetic and molecular characterization of the gill (NA+, K+)-ATPase from Macrobrachium amazonicum inhabitants of the Mato Grosso Pantanal and Tocantins-Araguaia River Basin
• Grantee: Francisco de Assis Leone
• Support Opportunities: Regular Research Grants