| Full text | |
| Author(s): |
Borges, Fernando A.
[1]
;
Rossini, Julhiano B.
[2]
;
Velludo, Patricia P.
[1]
;
Buzzulini, Carolina
[1]
;
Costa, Gustavo H.
[1]
;
Molento, Marcelo B.
[2, 3]
;
Costa, Alvimar J.
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Mato Grosso do Sul UFMS, Fac Med Vet & Zootecnia Famez, Dept Vet Med, BR-79070900 Campo Grande, MS - Brazil
[2] Univ Estadual Paulista Unesp, FCAV, Ctr Pesquisas Sanidade Anim Cppar, BR-14884900 Jaboticabal, SP - Brazil
[3] Univ Fed Parana, Lab Doencas Parasitarias, BR-80035050 Curitiba, Parana - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Pesquisa Veterinária Brasileira; v. 31, n. 9, p. 731-736, SEP 2011. |
| Web of Science Citations: | 3 |
| Abstract | |
Recent advances in anthelmintic resistant phenotype reversion by Pgp modulating drugs in ruminant nematodes indicate that this can be a useful tool to helminth control. The aim of the present study was to evaluate the efficacy of ivermectin (IVM) in combination with verapamil (VRP), in oil or water-based vehicle, against an IVM-resistant field isolate of Haemonchus contortus through a larval migration assay and experimental infection trial. In the in vitro assay was observed a phenotypic reversion of H. contortus resistance to ivermectin at a high concentration of VRP, increasing IVM efficacy from 53.1% to 94.3. In the in vivo trial, IVM + VRP demonstrated 36.02% efficacy compared to the 7.75% of IVM alone. The vehicle formulation showed no influence in efficacy. These are the first results demonstrating the effect of VRP as a partial IVM-resistance phenotype reverser in a field isolate of IVM-resistant H. contortus experimentally inoculated in sheep. (AU) | |