| Full text | |
| Author(s): |
Dias, Luana Mendonca
;
Cilli, Eduardo Maffud
;
Medeiros, Karine Sousa
;
Brasil, Maria Carolina Oliveira de Arruda
;
Marin, Lina Maria
;
Siqueira, Walter L.
;
Pavarina, Ana Claudia
Total Authors: 7
|
| Document type: | Journal article |
| Source: | MICROORGANISMS; v. 12, n. 1, p. 21-pg., 2024-01-01. |
| Abstract | |
The aim of the study was to investigate the effect of antimicrobial peptides (AMPs) Hylin-a1, KR-12-a5, and Temporin-SHa in Candida albicans as well as the biocompatibility of keratinocytes spontaneously immortalized (NOK-si) and human gingival fibroblasts (FGH) cells. Initially, the susceptible (CaS-ATCC 90028) and fluconazole-resistant (CaR-ATCC 96901) C. albicans strains were grown to evaluate the effect of each AMP in planktonic culture, biofilm, and biocompatibility on oral cells. Among the AMPs evaluated, temporin-SHa showed the most promising results. After 24 h of Temporin-SHa exposure, the survival curve results showed that CaS and CaR suspensions reduced 72% and 70% of cell viability compared to the control group. The minimum inhibitory/fungicide concentrations (MIC and MFC) showed that Temporin-SHa was able to reduce >= 50% at >= 256 mu g/mL for both strains. The inhibition of biofilm formation, efficacy against biofilm formation, and total biomass assays were performed until 48 h of biofilm maturation, and Temporin-SHa was able to reduce >= 50% of CaS and CaR growth. Furthermore, Temporin-SHa (512 mu g/mL) was classified as non-cytotoxic and slightly cytotoxic for NOK-si and FGH, respectively. Temporin-SHa demonstrated an anti-biofilm effect against CaS and CaR and was biocompatible with NOK-si and FGH oral cells in monolayer. (AU) | |
| FAPESP's process: | 21/06476-3 - Analysis of the peptide Temporin-SHa on Candida albicans biofilm and oral cavity cells |
| Grantee: | Karine Sousa Medeiros |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 22/12059-9 - Antimicrobial activity of salivary peptides against Candida albicans and Streptococcus mutans dual-species biofilm formed on acrylic resin |
| Grantee: | Luana Mendonça Dias Santana |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 13/07276-1 - CEPOF - Optics and Photonic Research Center |
| Grantee: | Vanderlei Salvador Bagnato |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery |
| Grantee: | Glaucius Oliva |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 20/16227-8 - Influence of photodynamic therapy combined with antimicrobial peptides on Candida albicans biofilm and 3D cell culture |
| Grantee: | Luana Mendonça Dias Santana |
| Support Opportunities: | Scholarships in Brazil - Doctorate |