Multiple PDT sessions with chlorin-e6 and LL-37 loaded-nanoemulsion provide limited benefits to periodontitis in rats

Author(s)

de Carvalho, Gabriel Garcia ; Vieira, Basilio Rodrigues ; Carvalho, Jhonatan de Souza ; de Sousa, Frederico Barbosa ; Cerri, Paulo Sergio ; de Oliveira, Kleber Thiago ; Chorilli, Marlus ; Zandim-Barcelos, Daniela Leal ; Spolidorio, Luis Carlos ; Spolidorio, Denise Madalena Palomari

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 49, p. 9-pg., 2024-09-23.

Abstract

Background: The combination of photodynamic therapy (PDT) and LL-37 has never been tested in an animal study and our research team background suggests this strategy might be a promising alternative to intensify periodontitis resolution. This study aimed to assess the effects of multiple sessions of PDT with chlorin-e6 conjugated to the antimicrobial peptide LL-37 loaded nanoemulsion, as adjunctive therapy in experimental periodontitis in rats. Methods: Experimental periodontitis was induced in 81 rats. After disease establishment, animals were assigned to three groups: SRP (scaling and root planning); SRP + 1PDT, SRP followed by a single PDT session; SRP + 4PDT (n n = 27), SRP followed by four PDT sessions at 0, 24, 48 and 72 h after SRP. Animals were subjected to euthanasia at 7, 14 and 28 days, and samples were submitted to osteoclast quantification, immunological and microtomography analysis. Results: All treatments resulted in significant periodontal improvements and there was no significant difference between the groups in both local inflammatory response and healing process. Minimal adjunctive effects could be found for the combined therapy in terms of cytokine levels (IL-1 beta and IL-10), with no statistical significance. However, the number of TRAP-positive osteoclasts per mm of alveolar bone linear surface for the group treated with PDT sessions was significantly lower than those treated with SRP only. Conclusions: Multiple PDT sessions with chlorin-e6 and LL-37 nanoemulsion as an adjunct to scaling and root planning reduced the presence of osteoclast in the local site but did not contribute towards bone regeneration and IL-1 beta and IL-10 levels. (AU)

• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 20/04499-3 - Chlorin-e6-mediated antimicrobial photodynamic therapy enhanced by nanoemulsion and antimicrobial peptide for the treatment of periodontitis
• Grantee: Gabriel Garcia de Carvalho
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 23/04020-8 - APIs synthesis using enabling technologies: approaches involving electrosynthesis and photocatalysis under continuous flow conditions
• Grantee: Kleber Thiago de Oliveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/09088-1 - Assessment of photodynamic therapy against polymicrobial acne biofilms: a preclinical study
• Grantee: Sarah Raquel de Annunzio
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 21/05881-1 - Development and evaluation of nanofibers and the nanostructured lipid carrier associated with hesperitin as a therapeutic potential in the repair of periodontal tissues
• Grantee: Denise Madalena Palomari Spolidorio
• Support Opportunities: Regular Research Grants