| Full text | |
| Author(s): |
Silva, Jessika C. A.
;
Marques-Neto, Lazaro M.
;
Carvalho, Eneas
;
Del Carpio, Alejandra M. G.
;
Henrique, Camila
;
Leite, Luciana C. C.
;
Mitsunari, Thais
;
Elias, Waldir P.
;
Munhoz, Danielle D.
;
Piazza, Roxane M. F.
Total Authors: 10
|
| Document type: | Journal article |
| Source: | TOXINS; v. 16, n. 11, p. 15-pg., 2024-11-01. |
| Abstract | |
The functions of bacterial plasmid-encoded toxin-antitoxin (TA) systems are unambiguous in the sense of controlling cells that fail to inherit a plasmid copy. However, its role in chromosomal copies is contradictory, including stress-response-promoting fitness and antibiotic treatment survival. A hybrid pathogenic Escherichia coli strain may have the ability to colonize distinct host niches, facing contrasting stress environments. Herein, we determined the influence of multiple environmental stress factors on the bacterial growth dynamic and expression profile of previously described TA systems present in the chromosome of a hybrid atypical enteropathogenic and extraintestinal E. coli strain. Genomic analysis revealed 26 TA loci and the presence of five type II TA systems in the chromosome. Among the tested stress conditions, osmotic and acid stress significantly altered the growth dynamics of the hybrid strain, enhancing the necessary time to reach the stationary phase. Using qPCR analyses, 80% of the studied TA systems were differentially expressed in at least one of the tested conditions, either in the log or in the stationary phase. These data indicate that type II TA systems may contribute to the physiology of pathogenic hybrid strains, enabling their adaptation to different milieus. (AU) | |
| FAPESP's process: | 17/14821-7 - Exploring novel virulence strategies in Escherichia coli |
| Grantee: | Tânia Aparecida Tardelli Gomes do Amaral |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 19/02305-0 - Investigation of immune response mechanisms effective of a BCG Vaccine Recombinant against Tuberculosis by Systems Biology |
| Grantee: | Lázaro Moreira Marques Neto |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 17/24832-6 - Development of vaccines based on recombinant BCG: Tuberculosis, Pertussis, Pneumococcus and Schistosoma |
| Grantee: | Luciana Cezar de Cerqueira Leite |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 23/01700-8 - Role of the type II toxin-antitoxin system in the bacterial pathogenesis of a hybrid strain: atypical enteropathogenic Escherichia coli and extraintestinal E. coli |
| Grantee: | Jessika Cristina Alves da Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |