| Full text | |
| Author(s): |
Caprioli, Bruna
;
Eichler, Rosangela A. S.
;
Silva, Renee N. O.
;
Martucci, Luiz Felipe
;
Reckziegel, Patricia
;
Ferro, Emer S.
Total Authors: 6
|
| Document type: | Journal article |
| Source: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 24, n. 20, p. 22-pg., 2023-10-01. |
| Abstract | |
Neurolysin oligopeptidase (E.C.3.4.24.16; Nln), a member of the zinc metallopeptidase M3 family, was first identified in rat brain synaptic membranes hydrolyzing neurotensin at the Pro-Tyr peptide bond. The previous development of C57BL6/N mice with suppression of Nln gene expression (Nln-/-), demonstrated the biological relevance of this oligopeptidase for insulin signaling and glucose uptake. Here, several metabolic parameters were investigated in Nln-/- and wild-type C57BL6/N animals (WT; n = 5-8), male and female, fed either a standard (SD) or a hypercaloric diet (HD), for seven weeks. Higher food intake and body mass gain was observed for Nln-/- animals fed HD, compared to both male and female WT control animals fed HD. Leptin gene expression was higher in Nln-/- male and female animals fed HD, compared to WT controls. Both WT and Nln-/- females fed HD showed similar gene expression increase of dipeptidyl peptidase 4 (DPP4), a peptidase related to glucagon-like peptide-1 (GLP-1) metabolism. The present data suggest that Nln participates in the physiological mechanisms related to diet-induced obesity. Further studies will be necessary to better understand the molecular mechanism responsible for the higher body mass gain observed in Nln-/- animals fed HD. (AU) | |
| FAPESP's process: | 16/04000-3 - Pharmacology of oligopeptidases and intracellular peptides |
| Grantee: | Emer Suavinho Ferro |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 19/25943-1 - Intracellular peptides as modulators of thermogenesis-related metabolic pathways |
| Grantee: | Patrícia Reckziegel |
| Support Opportunities: | Research Grants - Young Investigators Grants |