Caenorhabditis elegans (C. elegans) sample preparation for metabolomics and lipidomics analysis - A review

Author(s)

Santoro, Adriana Leandra ; Vendramini, Pedro H. ; Braga, Deisi L. ; Martins, Lucas Gelain ; Dos Santos, Gabriel F. ; Lima, Gesiane S. ; Tasic, Ljubica ; Mori, Marcelo A.

• Document type: Journal article
• Source: TRAC-TRENDS IN ANALYTICAL CHEMISTRY; v. 169, p. 11-pg., 2023-10-25.

Abstract

Caenorhabditis elegans (C. elegans) is a powerful model organism which has been extensively characterized at the genetic and cellular levels and used to study various biological processes. However, less is known about how metabolic pathways operate in C. elegans and how metabolites participate in worm's physiology. Metabolomics and lipidomics have emerged as valuable tools for understanding C. elegans biology and its association with physiological and pathological conditions in humans. Metabolomics and lipidomics methods are diverse and lead to different outcomes depending on a number of variables, including sample preparation, metabolite extraction, the method of detection and data analysis. In this Review, the main steps involved in C. elegans lipidomics and metabolomics analyses are presented, directing particular attention to the advantages and drawbacks of each method of sample preparation and processing in terms of simplicity and metabolite coverage, and also listing the most frequently used protocols and their applications. (AU)

• FAPESP's process: 13/07607-8 - OCRC - Obesity and Comorbidities Research Center
• Grantee: Licio Augusto Velloso
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 21/08354-2 - The interplay between the immune system and metabolism as a key determinant of the aging process
• Grantee: Marcelo Alves da Silva Mori
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/01184-9 - CAMeLEOm: cross-species analysis of metabolic, lifespan effects and omics of dietary restriction mimetics
• Grantee: Marcelo Alves da Silva Mori
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 20/06057-8 - Exploring new mechanisms of intercellular communication in metabolic diseases
• Grantee: Adriana Leandra Santoro
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/11792-9 - Characterization in C. elegans of novel genes involved in aging and the evolutionarily conserved mechanisms of dietary restriction mimetics
• Grantee: Deisi Lilian Braga Shimo
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)