| Full text | |
| Author(s): Show less - |
Souza, Luana Lelis
;
Dominguez-Castano, Pablo
;
Gianvecchio, Sarah Bernardes
;
Sakamoto, Leandro Sannomiya
;
Rodrigues, Gustavo Roberto Dias
;
Soares, Tainara Luana da Silva
;
Bonilha, Sarah Figueiredo Martins
;
Marcatto, Juliana de Oliveira Santos
;
Albuquerque, Lucia Galvao
;
Silva, Josineudson Augusto I. I. Vasconcelos
;
Mercadante, Maria Eugenia Zerlotti
Total Authors: 11
|
| Document type: | Journal article |
| Source: | JOURNAL OF ANIMAL SCIENCE; v. 102, p. 10-pg., 2024-07-27. |
| Abstract | |
The objectives of the present study were to estimate the heritability for daily methane emission (CH4 ) and residual daily methane emission (CH4 (res)) in Nellore cattle, as well as to perform genome-wide association studies (GWAS) to identify genomic regions and candidate genes influencing the genetic variation of CH4 and CH4 (res.). Methane emission phenotypes of 743 Nellore animals belonging to 3 breeding programs were evaluated. CH4 was measured using the sulfur hexafluoride (SF6) tracer technique (which involves an SF6 permeation tube introduced into the rumen, and an appropriate apparatus on each animal), and CH4res was obtained as the difference between observed CH4 and CH4 adjusted for dry matter intake. A total of 6,252 genotyped individuals were used for genomic analyses. Data were analyzed with a univariate animal model by the single-step GBLUP method using the average information restricted maximum likelihood (AIREML) algorithm. The effects of single nucleotide polymorphisms (SNPs) were obtained using a single-step GWAS approach. Candidate genes were identified based on genomic windows associated with quantitative trait loci (QTLs) related to the 2 traits. Annotation of QTLs and identification of candidate genes were based on the initial and final coordinates of each genomic window considering the bovine genome ARS-UCD1.2 assembly. Heritability estimates were of moderate to high magnitude, being 0.42 +/- 0.09 for CH4 and 0.21 +/- 0.09 for CH4 (res), indicating that these traits will respond rapidly to genetic selection. GWAS revealed 11 and 15 SNPs that were significantly associated (P < 10(-6)) with genetic variation of CH4 and CH4 res, respectively. QTLs associated with feed efficiency, residual feed intake, body weight, and height overlapped with significant markers for the traits evaluated. Ten candidate genes were present in the regions of significant SNPs; 3 were associated withCH(4) and 7 with CH4 (res.) The identified genes are related to different functions such as modulation of the rumen microbiota, fatty acid production, and lipid metabolism. CH4 and CH4 (res) presented sufficient genetic variation and may respond rapidly to selection. Therefore, these traits can be included in animal breeding programs aimed at reducing enteric methane emissions across generations. (AU) | |
| FAPESP's process: | 17/10630-2 - Genetic aspects of meat production quality, efficiency and sustainability in Nelore breed animals |
| Grantee: | Lucia Galvão de Albuquerque |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 18/20026-8 - Multi-user equipment approved in grant 2017/10630-2: server |
| Grantee: | Lucia Galvão de Albuquerque |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 23/11176-4 - Mapping candidates for lethal haplotypes and genomic regions associated with the occurrence of abortion and pre-weaning mortality in Nellore cattle |
| Grantee: | Gustavo Roberto Dias Rodrigues |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 17/50339-5 - Institutional research development plan of the Animal Science Institute (PDIp) |
| Grantee: | Lenira El Faro Zadra |
| Support Opportunities: | Research Grants - State Research Institutes Modernization Program |
| FAPESP's process: | 18/17313-5 - Enteric methane emission, feed efficiency and digestibility of Nellore cattle |
| Grantee: | Leandro Sannomiya Sakamoto |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 19/11738-7 - Phenotypic, genetic correlations and genomic wide association between feed efficiency and enteric methane emission of Nellore cattle |
| Grantee: | Luana Lelis Souza |
| Support Opportunities: | Scholarships in Brazil - Doctorate |