In vivo analysis of endogenous hyaluronic acid in the dermis by confocal Raman spectroscopy after the application of a dermocosmetic

Author(s)

Medeiros Neto, Lazaro Pinto ; de Santana, Felipe Bachion ; de Almeida, Ritiane Modesto ; Magalhaes, Wagner Vidal ; Mitsutake, Hery ; Barbosa, Miguel Brito ; Martin, Airton Abrahao

• Document type: Journal article
• Source: BIOMEDICAL SPECTROSCOPY, MICROSCOPY, AND IMAGING III; v. 13006, p. 10-pg., 2024-01-01.

Abstract

Evaluating endogenous hyaluronic acid (HA) in the skin is essential to understanding the ageing process and analysing the effectiveness of dermocosmetics. In this sense, this study evaluated the skin of 10 individuals between 51 and 65 years old before and after applying a dermocosmetic to stimulate HA production. For this, Gen2 confocal Raman (Rivers Diagnostic) coupled to a 785 nm laser was used. Participants underwent a 7-day washout and then had their skin measured (T0 - control). After the control measure, they used the product for 60 days, applying it twice a day (morning and evening). Measurements were made at times T0, T30 and T60 days in the spectral range of 400 to 1800 cm(-1). Spectral data was processed by removing the baseline, smoothing and vector normalization. The T30 and T60 spectra were subtracted from the control measurement (T0) and compared with the second derivative spectrum of hyaluronic acid. It was observed that the main peaks of HA were presented in the difference between the spectra before and after using the dermocosmetic. The main peaks observed were 1076, 1084, 1096, 1126, 1132 and 1208 cm-1. The difference between these peaks increased after 30 and 60 days of using the dermocosmetic product, indicating an increase in the concentration of HA in the skin. Therefore, the in vivo assessment of cutaneous HA by confocal Raman spectroscopy can be used to understand the role of dermocosmetics in skin ageing, allowing for more effective treatments. (AU)

• FAPESP's process: 21/14696-3 - In vivo and non-invasive detection of the effects of the use of products on hyaluronic acid in human skin
• Grantee: Lázaro Pinto Medeiros Neto
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• FAPESP's process: 22/13020-9 - In vivo and non-invasive detection of the effects of the use of products on hyaluronic acid in human skin.
• Grantee: Ritiane Modesto de Almeida Moreira
• Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training
• FAPESP's process: 20/00928-7 - Development of an in vivo and non-invasive process for quantifying hyaluronic acid in human skin
• Grantee: Lázaro Pinto Medeiros Neto
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE