| Full text | |
| Author(s): Show less - |
Reche, Daniela Terra de-Apoena
;
Machado, Nicolle Rakanidis
;
Fagundes, Beatriz Oliveira
;
Bergamasco, Isabella Siuffi
;
de Sousa, Thamires Rodrigues
;
do Nascimento, Lais Alves
;
Cunha, Fernando Roberto Machado
;
de-Oliveira, Marilia Garcia
;
Sgnotto, Fabio da-Ressureicao
;
Franca, Carolina Nunes
;
Victor, Jefferson Russo
Total Authors: 11
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| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 14, n. 1, p. 11-pg., 2024-03-27. |
| Abstract | |
Studies about thymic B cells are scarce in the literature, but it was suggested that they can exert modulatory and regulatory functions on the immune system. Thymic B cells can play some role in regulating the most frequent allergic background worldwide, the atopy induced by the mite Dermatophagoides pteronyssinus (Der p). Here, we aimed to evaluate if the polyclonal IgG repertoire produced by Der p-atopic individuals can influence the homing and cytokine profile of human thymic B derived from non-atopic children aged less than seven days. With this purpose, we produced polyclonal IgG formulations and cultivated human thymocytes in their presence. We also assessed IgG subclasses and the direct interaction of IgG with thymic B cell membranes. Our results could demonstrate that Der p-atopic IgG could not reduce the expression of alpha 4 beta 7 homing molecule as observed in response to the other IgG formulations and could reduce the frequency of IFN-gamma- and IL-9-producing thymic B cells compared to the mock condition. Der p-atopic IgG could also induce thymic IL-10-producing B cells compared to control conditions. The IgG derived from Der p-atopic individuals failed to diminish the population of IL-13-producing thymic B cells, unlike the reduction observed with other IgG formulations when compared to the mock condition. All IgG formulations had similar levels of IgG subclasses and directly interacted with thymic B cell membranes. Finally, we performed experiments using peripheral non-atopic B cells where IgG effects were not observed. In conclusion, our observation demonstrates that IgG induced in allergic individuals can modulate non-atopic thymic B cells, potentially generating thymic B cells prone to allergy development, which seems to not occur in mature B cells. (AU) | |
| FAPESP's process: | 21/08225-8 - The Hooks without bait theory: mechanisms involved in the immunomodulatory effect of IgG on the thymic modulation of TCD4, TCD8, gdT, iNKT MAIT and B cells, focus on atopic diseases and COVID-19 |
| Grantee: | Jefferson Russo Victor |
| Support Opportunities: | Research Grants - Young Investigators Grants |