Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins

Author(s)

Bermudez-Puga, Sebastain ; Dias, Meriellen ; Reis, Iara Lima ; de Oliveira, Taciana Freire ; de Almeida, Sonia Regina Yokomizo ; Mendes, Maria Anita ; Moore, Simon J. ; Almeida, Jose R. ; Proano-Bolanos, Carolina ; de Souza Oliveira, Ricardo Pinheiro

• Document type: Journal article
• Source: Biochimie; v. 225, p. 8-pg., 2024-06-06.

Abstract

Listeria monocytogenes is a human opportunistic foodborne pathogen that produces life-threatening infections with a high mortality rate. The control of Listeria in the food production environment and effective clinical management of human listeriosis are challenging due to the emergence of antibiotic resistance. Hence we evaluate the in vitro anti-Listeria activity of two synthetic cruzioseptins reproducing their natural sequences CZS-9, and CZS-12, and one engineered sequence based on CZS-1, named [K4K15] CZS-1. The assessment of the in vitro potential of cruzioseptins, highlighted the promising antibacterial effect of [K4K15]CZS-1 in very low concentrations (0.91 mM) and its thermal stability at high-temperature conditions, is compatible with the food industry. Microscopic and metabolomic analyses suggest cruzioseptin induces anti-Listeria bioactivity through membrane disruption and changes in the intracellular metabolome. We also report that [K4K15]CZS-1 is not resistant to peptidases/proteases emphasizing a key advantage for their use as a food preservative. However, there is a need for further structural and functional optimisations for the potential clinical application as an antibiotic. In conclusion, [K4K15]CZS1 stand out as membrane-active peptides with the ability to induce shifts in the bacteria metabolome and inspire the development of strategies for the prevention of L. monocytogenes emergence and dissemination. (c) 2024 Elsevier B.V. and Soci & eacute;t & eacute; Fran & ccedil;aise de Biochimie et Biologie Mol & eacute;culaire (SFBBM). All rights are reserved, including those for text and data mining, AI training, and similar technologies. (AU)

• FAPESP's process: 18/25511-1 - Bioprospecting of bacteriocin-producing bacteria: from culture optimization to the application in animal production systems
• Grantee: Ricardo Pinheiro de Souza Oliveira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 20/15599-9 - Mult-user Equipment approved in grant 2018/25511-1: Semi-Preparative High Pressure Liquid Chromatography
• Grantee: Ricardo Pinheiro de Souza Oliveira
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 22/02936-2 - Probiotics and bacteriocins in Nile Tilapia feeding: effects on growth performance, immunology and experimental infection
• Grantee: Sebastián Armando Bermúdez Puga
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)