Analysis of the spread of bacterial resistance after photodynamic inactivation by minimum inhibitory concentration (MIC)

Author(s)

De Lima, R. V. ; Soares, J. M. ; Blanco, K. C. ; Bagnato, V. S.

• Document type: Journal article
• Source: PHOTONIC DIAGNOSIS, MONITORING, PREVENTION, AND TREATMENT OF INFECTIONS AND INFLAMMATORY DISEASES 2024; v. 12822, p. 5-pg., 2024-01-01.

Abstract

The objective of this study was to prepare mixed cultures of both sensitive and resistant bacteria of the same species in varying proportions (20% and 80%, 50% and 50% and 80% and 20%) We aimed to analyze the minimum inhibitory concentration (MIC) both before and after photoinactivation treatment. This treatment involved the use of the synthetic photosensitizer curcumin 5 mM (PDTPharma (R)) and irradiation with an LED lighting device (Biotable (R) - produced by MM Optics-Brasil) emitting a wavelength of 450 nm, at 40 mW/cm(2), and a light dose of 10 J/cm(2). The purpose was to examine the impact of photodynamic action on the spread of bacterial resistance. Combating the proliferation of antimicrobial resistance is a critical global health issue, necessitating treatment methods that adequately address this challenge. (AU)

• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Projects - Thematic Grants