An alternative method for comparing hemorrhagic activity of snake venoms following one of the 3R's principles

Author(s)

Tasima, Lidia Jorge ; Kavazoi, Victor Koiti ; de Lima, Eduardo Oliveira Venancio ; Grego, Kathleen Fernandes ; Tanaka-Azevedo, Anita Mitico

• Document type: Journal article
• Source: Toxicon; v. 250, p. 4-pg., 2024-09-28.

Abstract

Venom-induced hemorrhage analysis usually is performed by Minimum Hemorrhagic Dose (MHD), however a similar method can be used to compare venoms with fewer laboratory animals. Our work compared the MHD of five different venoms, with the size of hemorrhagic spot, finding good correlations in the results. Considering the 3Rs principle, we propose the use of the hemorrhagic spot method to compare hemorrhagic activity of snake venoms, rather than using the MHD method, since the first one needs 5 times less animals than the other. (AU)

• FAPESP's process: 21/04287-9 - Correlation between in vitro assays and in vivo assays: search for approaches in the study of snake venoms that dispense with the use of animals following the 3R's principle
• Grantee: Lidia Jorge Tasima
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 22/15660-5 - Ontogenetic study of the venom of Bothrops alternatus snakes and analysis of phospholipase A2 inhibitors as a complementary therapy to serum therapy in bothropic accidents
• Grantee: Eduardo Oliveira Venancio de Lima
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 21/11707-4 - Evaluation of alternative in vitro methods to the use of animals for in vivo tests in the study of snake venoms: principle of the 3R's
• Grantee: Anita Mitico Tanaka-Azevedo
• Support Opportunities: Regular Research Grants