Concentration of stigmasterol, β-sitosterol and squalene from passion fruit (Passiflora edulis Sims.) by-products by supercritical CO 2 adsorption in zeolite 13-X

Author(s)

dos Santos, Luana C. ; Scopel, Eupidio ; Hatami, Tahmasb ; Rezende, Camila A. ; Martinez, Julian

• Document type: Journal article
• Source: JOURNAL OF SUPERCRITICAL FLUIDS; v. 209, p. 9-pg., 2024-03-27.

Abstract

Phytosterols and squalene, widely applied in pharmaceutical and food industries, were extracted and concentrated from passion fruit by-products (PFBP). A supercritical extraction using CO 2 (SFE) was performed for PFBP, and the obtained extract underwent supercritical fractionation (SFA) (inlet at 35 MPa, 50 - 70 degrees C) in a column packed with zeolite 13-X (5 - 15 g). The extraction and fractionation yields were measured, whereas the composition of the PFBP extract and their fractions was assessed by gas chromatography -mass spectrometry (GCMS). Fractionated extract recovered presented different trends depending on temperature and zeolite mass combinations, and the highest recovery (approximately 95%) was achieved at 50 degrees C with 15 g of adsorbent. At the same SFA conditions, the concentration of squalene, beta -sitosterol and stigmasterol was twice that obtained in PFBP extract. Finally, SFA proved to be a clean and effective technique to concentrate squalene and phytosterols from PFBP, which should be extended to other food residues. (AU)

• FAPESP's process: 19/19360-3 - Plant cell wall-inspired nanocomposite hydrogels for biomedical applications
• Grantee: Eupídio Scopel
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 21/12071-6 - Tailoring colloids through supramolecular interactions: from fundamentals to applications
• Grantee: Watson Loh
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 21/01008-1 - Supercritical technology in the reverse engineering context: formulation of new products from citrus agro-industrial residues
• Grantee: Julian Martínez
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/20700-0 - Fractionation of passion fruit (Passiflora edulis) seed oil using adsorption process with zeolite NA13X
• Grantee: Renata Gonçalves Broco e Silva
• Support Opportunities: Scholarships in Brazil - Scientific Initiation