Biorefinery of brewery spent grain to obtain bioproducts with high value-added in the market

Author(s)

Outeirino, David ; Costa-Trigo, Ivan ; Ochogavias, Aida ; Oliveira, Ricardo Pinheiro de Souza ; Guerra, Nelson Perez ; Salgado, Jose Manuel ; Dominguez, Jose Manuel

• Document type: Journal article
• Source: NEW BIOTECHNOLOGY; v. 79, p. 9-pg., 2024-01-02.

Abstract

The brewery industry is under economic and environmental pressure to minimize residual management costs, particularly brewery spent grain (BSG), which accounts for 80-85% (w/w) of the total by-products generated. BSG is a lignocellulosic material primarily composed of carbohydrates, proteins and lipids. Developing a bio-refinery model for conversion of BSG into value-added products is a plausible idea. Previous work optimized the pretreatment of BSG with the ionic liquid [N1112OH][Gly] and further release of fermentable sugar-containing solutions by enzymatic hydrolysis, using an enzymatic cocktail obtained by solid-state fermentation of BSG with Aspergillus brasiliensis CECT 2700 and Trichoderma reesei CECT 2414. The current work ends the biorefinery process, studying the fermentation of these culture media with two LAB strains, Lactobacillus pentosus CECT 4023 and Lactobacillus plantarum CECT 221, from which the production of organic acids, bacteriocins, and microbial biosurfactants (mBS) was obtained. In addition to the bacteriocin activity observed, a mass balance of the whole biorefinery process quantified the production of 106.4 g lactic acid and 6.76 g mBS with L. plantarum and 116.1 g lactic acid and 4.65 g mBS with L. pentosus from 1 kg of dry BSG. Thus, BSG shows a great potential for waste valorization, playing a major role in the implementation of biomass biorefineries in circular bioeconomy. (AU)

• FAPESP's process: 18/25511-1 - Bioprospecting of bacteriocin-producing bacteria: from culture optimization to the application in animal production systems
• Grantee: Ricardo Pinheiro de Souza Oliveira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 23/09256-0 - Valorization of biomass residues in a biorefinery concept for Brazilian industry's circular economy transition
• Grantee: Ricardo Pinheiro de Souza Oliveira
• Support Opportunities: Research Grants - Visiting Researcher Grant - International