Labeled extracellular vesicles can be found in the blood plasma shortly after intrauterine infusion in bovine

Author(s):Show less -

Fiorenza, Mariani Farias ; Bridi, Alessandra ; dos Santos, Gislaine ; Rosa, Paola Maria ; Alves, Luana ; Ferst, Juliana Germano ; Ferraz, Priscila Assis ; Pugliesi, Guilherme ; Pohler, Ky ; Perecin, Felipe ; Meirelles, Flavio Vieira ; da Silveira, Juliano Coelho

• Document type: Journal article
• Source: ANIMAL REPRODUCTION; v. 21, n. 3, p. 6-pg., 2024-01-01.

Abstract

This study explored the migration of follicular fluid (FF)-derived extracellular vesicles (EVs) of the uterine environment to the bloodstream and their interaction with neutrophils in vivo and in vitro. For the in vivo experiment, six Nellore heifers (Bos indicus) received an intrauterine infusion seven days after ovulation with 1X PBS only (sham group; n=1), 1X PBS stained with lipophilic dye PKH26 (control group; n=2), or FFderived EVs stained with PKH26 (treated group; n=3). Plasma was collected at 0, 10, 30, 60-, 180-, 360-, 720-, and 1440-min post-infusion to obtained EVs for analysis by nano flow cytometry. Labeled EVs were present in the bloodstream at 30- and 60-min post-infusion in the treatment group. Additionally, plasma derived-EVs from all groups were positive for Calcein-AM, Alix, Syntenin, and Calnexin, which confirm the presence of EVs. The second experiment utilized the plasma-derived EVs from the heifers from 30 and 60 min timepoints to evaluate if neutrophils can uptake EVs in vitro. As results, it was possible to observe the presence of labeled EVs in neutrophils treated with plasma derived-EVs from the treatment group. In summary, our results suggest that labeled EVs can migrate from the uterine environment rapidly and interact with circulating immune cells in bovine. (AU)

• FAPESP's process: 22/01505-8 - Multi-user equipment approved in grant 21/06645-0: CytoFLEX System B3-R0-V3
• Grantee: Juliano Coelho da Silveira
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 19/06696-3 - International consortium for the study of serum extracellular vesicles during early embryonic development of livestock animals
• Grantee: Juliano Coelho da Silveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 22/01235-0 - Extracellular vesicles as an amplifier of embryonic signals: implications to maternal immune response
• Grantee: Mariani Farias Fiorenza
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 22/01433-7 - Multi-user equipment approved in the grant FAPESP 21/06645-0: Inverted Microscope Yhunder Imager 3D Assay
• Grantee: Juliano Coelho da Silveira
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 21/06645-0 - Extracellular vesicles as a platform for diagnostic and manipulation of the in vitro embryo production system: the next generation in animal reproduction
• Grantee: Juliano Coelho da Silveira
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2