| Full text | |
| Author(s): Show less - |
Gimenes, Gabriela Mandu
;
Pereira, Joice Naiara Bertaglia
;
da Silva, Eliane Borges
;
dos Santos, Alef Aragao Carneiro
;
Rodrigues, Thais Martins
;
Santana, Giovanna de Oliveira
;
Scervino, Maria Vitoria Martins
;
Pithon-Curi, Tania Cristina
;
Hirabara, Sandro Massao
;
Gorjao, Renata
;
Curi, Rui
Total Authors: 11
|
| Document type: | Journal article |
| Source: | CELLS; v. 13, n. 19, p. 15-pg., 2024-10-01. |
| Abstract | |
Diabetes mellitus is associated with changes in intestinal morphology and the enteric nervous system. We previously reported constipation in Goto-Kakizaki (GK) rats, a non-obese model for type 2 diabetes mellitus. Aim: The morpho-quantitative analysis of myenteric plexus neurons in the small and large intestines of 120-day-old male GK rats was investigated. Methods: The diabetes was confirmed by high fasting blood glucose levels. The myenteric plexus was evaluated through wholemount immunofluorescence. The morpho-quantitative analyses included evaluating neuronal density (neurons per ganglion) of the total neuronal population, the cholinergic and nitrergic subpopulations, and enteric glial cells per ganglion. The cell body area of 100 neurons per segment per animal was measured. Results: The total neurons and nitrergic subpopulation were unaltered in the GK rats' small and large intestines. The cholinergic subpopulation exhibited decreased density in the three segments of the small intestine and an increased number in the proximal colon of the GK rats. The number of enteric glial cells increased in the ileum of the GK rats, which could indicate enteric gliosis caused by the intestinal inflammatory state. The area of the cell body was increased in the total neuronal population of the jejunum and ileum of the GK rats. Frequency histograms of the cell body area distribution revealed the contribution of cholinergic neurons to larger areas in the jejunum and nitrergic neurons in the ileum. Conclusion: The constipation previously reported in GK rats might be explained by the decrease in the density of cholinergic neurons in the small intestine of this animal model. (AU) | |
| FAPESP's process: | 19/01942-6 - Neurochemical characterization of mioenteric plexus of Goto-Kakizaki rat intestine |
| Grantee: | Gabriela Mandu Gimenes |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 18/09868-7 - Cellular and molecular mechanisms of insulin resistance and inflammation in obese Wistar rats and lean Goto-Kakizaki rats: causes and associations with diet and physical exercise |
| Grantee: | Rui Curi |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 22/11249-9 - Slow intestinal transit and intestinal microbiota composition in the genesis of insulin resistance in Goto-Kakizaki rats |
| Grantee: | Gabriela Mandu Gimenes |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |