Biochemical and pharmacological characterization of PhTX-I a new myotoxic phospholipase A(2) isolated from Porthidium hyoprora snake venom

Author(s)

Huancahuire-Vega, Salomon ^[1] ; Ponce-Soto, Luis Alberto ^[1] ; Martins-de-Souza, Daniel ^[2] ; Marangoni, Sergio ^[1]

Affiliation

^[1] State Univ Campinas UNICAMP, Dept Biochem, Inst Biol, BR-13083970 Campinas, SP - Brazil

^[2] Max Planck Inst Psychiat, D-80804 Munich - Germany

• Document type: Journal article
• Source: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY; v. 154, n. 2, p. 108-119, AUG 2011.
• Web of Science Citations: 10

Abstract

This paper reports the biochemical and pharmacological characterization of a new myotoxic PLA(2) (EC 3.1.1.4) called PhTX-I, purified from Porthidium hyoprora venom by one step analytical chromatography reverse phase HPLC. The homogeneity of the PhTX-I fraction and its molecular mass were initially evaluated by SDS-PAGE and confirmed by MALDI-TOF spectrometry, indicating a molecular mass of 14.249 Da and constituted of a single polipeptidic chain. Amino acid sequence was determined by ``de novo sequencing,{''} in tandem mass spectrometry, belonging to D49-PIA(2) enzyme class and exhibiting high identity (44-90%) with other myotoxics PLA(2) from snake venoms. The enzymatic investigation showed maximal activity at pH 8 and 35-45 degrees C. This activity was dependent on Ca(2+), other cations (Mg(2+), Mn(2+), Cd(2+) and Zn(2+)) reduced notably the enzymatic activity, suggesting that the arrangement of the catalytic site presents an exclusive structure for Ca(2+). Ex vivo, whole venom and PhTX-I PLA(2) caused blockade of the neuromuscular transmission in young chick biventer cervicis preparations similar to other isolated snake venom toxins from the Bothrops genus. In vivo, both induced local myotoxicity and systemic interleukin-6 response upon intramuscular injection, additionally, induced moderate footpad edema. In vitro, both induced low cytotoxicity in skeletal muscle myoblasts, however PhTX-I PLA(2) was able to lyse myotubes. (C) 2011 Elsevier Inc. All rights reserved. (AU)