| Full text | |
| Author(s): Show less - |
Couto-Lima, Carlos Antonio
;
Machado, Maiaro Cabral Rosa
;
Anhezini, Lucas
;
Oliveira, Marcos Tulio
;
Molina, Roberto Augusto da Silva
;
da Silva, Rodrigo Ribeiro
;
Lopes, Gabriel Sarti
;
Trinca, Vitor
;
Colon, David Fernando
;
Peixoto, Pablo M.
;
Monesi, Nadia
;
Alberici, Luciane Carla
;
Ramos, Ricardo Guelerman P.
;
Espreafico, Enilza Maria
Total Authors: 14
|
| Document type: | Journal article |
| Source: | BIOMOLECULES; v. 14, n. 10, p. 18-pg., 2024-10-01. |
| Abstract | |
EMC1 is part of the endoplasmic reticulum (ER) membrane protein complex, whose functions include the insertion of transmembrane proteins into the ER membrane, ER-mitochondria contact, and lipid exchange. Here, we show that the Drosophila melanogaster EMC1 gene is expressed in the somatic musculature and the protein localizes to the sarcoplasmic reticulum (SR) network. Muscle-specific EMC1 RNAi led to severe motility defects and partial late pupae/early adulthood lethality, phenotypes that are rescued by co-expression with an EMC1 transgene. Motility impairment in EMC1-depleted flies was associated with aberrations in muscle morphology in embryos, larvae, and adults, including tortuous and misaligned fibers with reduced size and weakness. They were also associated with an altered SR network, cytosolic calcium overload, and mitochondrial dysfunction and dysmorphology that impaired membrane potential and oxidative phosphorylation capacity. Genes coding for ER stress sensors, mitochondrial biogenesis/dynamics, and other EMC components showed altered expression and were mostly rescued by the EMC1 transgene expression. In conclusion, EMC1 is required for the SR network's mitochondrial integrity and influences underlying programs involved in the regulation of muscle mass and shape. We believe our data can contribute to the biology of human diseases caused by EMC1 mutations. (AU) | |
| FAPESP's process: | 09/54014-7 - Acquisition of a biophotonic imaging system and a multiphoton microscopy system for in vivo imaging |
| Grantee: | Enilza Maria Espreafico |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 18/04017-9 - Genomic and functional characterization of putative melanoma-restricted lncRNAs (RMELs) |
| Grantee: | Enilza Maria Espreafico |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/16666-2 - Molecular and functional analysis of the gene CG2943, the gene KIAA0090 ortholog in Drosophila melanogaster |
| Grantee: | Carlos Antonio Couto Lima |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 22/05632-4 - Genetic and metabolic interactions among lactate dehydrogenase, glycerol phosphate dehydrogenase and alternative oxidase |
| Grantee: | Carlos Antonio Couto Lima |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 12/17890-6 - Involvement of KIAA0090 gene in UPR-ERAD responses, autophagy and apoptosis, and effects of its knock down on the proteome of melanoma cells and melanocytes |
| Grantee: | Rodrigo Ribeiro da Silva |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 21/10032-3 - Unraveling the oncogenic mechanisms of the lncRNA RMEL3 and its potential as therapeutic target |
| Grantee: | Enilza Maria Espreafico |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 21/06711-2 - Modulation of tissue growth and biomass accumulation by the mitochondrial alternative oxidase |
| Grantee: | Marcos Túlio de Oliveira |
| Support Opportunities: | Research Grants - Young Investigators Grants - Phase 2 |
| FAPESP's process: | 18/10089-2 - Neural, hormonal and nutritional control of autophagy |
| Grantee: | Isis Do Carmo Kettelhut |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/18189-5 - Studying a possible functional circuitry involving microRNAs, MITF, MYO5A/DLC2, RAB27A and connections with the invasion and metastasis cascade |
| Grantee: | Enilza Maria Espreafico |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/21242-2 - Molecular studies of the gene CG2943 in the development of normal structures and tumors induced by genetic manipulation in Drosophila melanogaster |
| Grantee: | Carlos Antonio Couto Lima |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 15/13396-5 - Drosophila melanogaster as a model for the study of cancer and degenerative diseases: The functional study of DmelEMC1 gene and the genetic interactions with other EMC complex members |
| Grantee: | Maiaro Cabral Rosa Machado |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 10/17259-9 - Studies on mechanisms of mitochondrial uncoupling by non-esterified fatty acids as a strategy for prevention / treatment of obesity |
| Grantee: | Luciane Carla Alberici |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 10/11812-8 - Study of the role of KIAA0090 protein in neoplastic transformation and cancer therapeutic resistance |
| Grantee: | Roberto Augusto Silva Molina |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 16/25325-8 - The dipteran protoracic glands: morphological, genomic and functional studies in the dipteran B. hygida and D. melanogaster |
| Grantee: | Nadia Monesi |
| Support Opportunities: | Regular Research Grants |