NPPC and AREG supplementation in IVM systems alter mRNA translation and decay programs-related gene expression in bovine COC

Author(s)

Saraiva, Helena Fabiana Reis de Almeida ; Sangalli, Juliano Rodrigues ; Alves, Luana ; da Silveira, Juliano Coelho ; Meirelles, Flavio Vieira ; Perecin, Felipe

• Document type: Journal article
• Source: ANIMAL REPRODUCTION; v. 21, n. 2, p. 19-pg., 2024-01-01.

Abstract

During oocyte meiosis resumption, a coordinated program of transcript translation and decay machinery promotes a remodeling of mRNA stores, which determines the success of the acquisition of competence and early embryo development. We investigated levels of two genes related to mRNA translation (CPEB1 and CPEB4) and two related to mRNA degradation (CNOT7 and ZFP36L2) machinery and found ZFP36L2 downregulated in in vitro-matured bovine oocytes compared to in vivo counterparts. Thereafter, we tested the effects of a pre-IVM step with NPPC and a modified IVM with AREG on the modulation of members of mRNA translation and degradation pathways in cumulus cells and oocytes. Our data showed a massive upregulation of genes associated with translational and decay processes in cumulus cells, promoted by NPPC and AREG supplementation, up to 9h of IVM. The oocytes were less affected by NPPC and AREG, and even though ZFP36L2 transcript and protein levels were downregulated at 9 and 19h of IVM, only one (KDM4C) from the ten target genes evaluated was differently expressed in these treatments. These data suggest that cumulus cells are more prone to respond to NPPC and AREG supplementation in vitro, regarding translational and mRNA decay programs. Given the important nursing role of these cells, further studies could contribute to a better understanding of the impact of these modulators in maternal mRNA modulation and improve IVM outcomes. (AU)

• FAPESP's process: 21/08759-2 - Messages stored in the bovine male gamete: effects of heat stress, role of epididymis-spermatozoa interactions and the paternal contribution to embryo development
• Grantee: Felipe Perecin
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
• Grantee: Dimas Tadeu Covas
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 22/01433-7 - Multi-user equipment approved in the grant FAPESP 21/06645-0: Inverted Microscope Yhunder Imager 3D Assay
• Grantee: Juliano Coelho da Silveira
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 21/09886-8 - Epigenetic maturation of the oocyte and totipotence: the potential of the oocyte and its relationship with the ovarian follicular niche
• Grantee: Lawrence Charles Smith
• Support Opportunities: Research Projects - SPEC Program
• FAPESP's process: 21/06645-0 - Extracellular vesicles as a platform for diagnostic and manipulation of the in vitro embryo production system: the next generation in animal reproduction
• Grantee: Juliano Coelho da Silveira
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2