| Full text | |
| Author(s): |
Medina, Deyber Arley Vargas
;
Lancas, Fernando Mauro
Total Authors: 2
|
| Document type: | Journal article |
| Source: | JOURNAL OF CHROMATOGRAPHY OPEN; v. 6, p. 14-pg., 2024-07-02. |
| Abstract | |
Miniaturized LC, encompassing capillary and nanoLC, presents significant analytical advantages in enhanced chromatographic performance and detectability and delivers considerable environmental and economic benefits. Despite these advantages, its utilization has primarily remained confined to omics sciences, with limited adoption in the routine targeted analysis of small molecules despite the lack of practical limitations. Initially, miniaturized LC encountered challenges related to scale reduction, necessitating the handling of minute sample volumes and lower flow rates. However, these obstacles have largely been overcome, with various instrumental platforms, columns, and consumables now available for efficient separations at the miniaturized scale. Moreover, the miniaturization of instruments facilitated by capillary and nanoLC has given rise to various portable LC platforms for in-situ analysis in recent years. Currently, researchers are exploring their applicability for diverse analytical purposes. However, the transition from conventional HPLC to miniaturized LC has been slow, and the technical factors limiting this shift still need to be clarified. Limited access to technology is among the primary obstacles identified, as transitioning may entail replacing conventional instruments with miniaturized versions. Additionally, a lack of proper training and background to handle small volumes and flow rates has hindered the widespread adoption of capillary and nano-LC. Notably, expertise in HPLC only sometimes translates to proficiency at the miniaturized scale. Through this review, we aim to facilitate the dissemination of miniaturized LC by providing readers with a comprehensive overview of its current state, showcasing recent advancements, and highlighting remarkable applications beyond omics sciences. (AU) | |
| FAPESP's process: | 23/02368-7 - Mechatronic aspects in the design and construction of an environmentally friendly system for capillary (cLC) and nano (nanoLC) liquid chromatography |
| Grantee: | Edwin Martin Cardenas Contreras |
| Support Opportunities: | Scholarships in Brazil - Technical Training Program - Technical Training |
| FAPESP's process: | 23/02154-7 - Design and construction of an environmentally friendly system for liquid chromatography on capillary (CLC) and nano (NanoLC) |
| Grantee: | Deyber Arley Vargas Medina |
| Support Opportunities: | Scholarships in Brazil - Innovative Research in Small Business - PIPE |
| FAPESP's process: | 22/07787-5 - Design and construction of an environmentally friendly system for capillary (cLC) and nano (nanoLC) liquid chromatography |
| Grantee: | Deyber Arley Vargas Medina |
| Support Opportunities: | Research Grants - Innovative Research in Small Business - PIPE |