Embryotoxicity Induced by Triclopyr in Zebrafish (Danio rerio) Early Life Stage

Author(s)

Bertoni, Italo ; Sales, Bianca Camargo Penteado ; Viriato, Cristina ; Peixoto, Paloma Vitoria Lima ; Pereira, Lilian Cristina

• Document type: Journal article
• Source: TOXICS; v. 12, n. 4, p. 15-pg., 2024-04-01.

Abstract

Triclopyr, an auxin-like herbicide that is widely employed for managing weeds in food crops and pastures, has been identified in various environmental settings, particularly aquatic ecosystems. Limited understanding of the environmental fate of this herbicide, its potential repercussions for both the environment and human health, and its insufficient monitoring in diverse environmental compartments has caused it to be recognized as an emerging contaminant of concern. In this study, we have investigated how triclopyr affects zebrafish, considering a new alternative methodology. We focused on the endpoints of developmental toxicity, neurotoxicity, and behavior of zebrafish embryos and larvae. We determined that triclopyr has a 96 h median lethal concentration of 87.46 mg/L (341.01 mu M). When we exposed zebrafish embryos to sublethal triclopyr concentrations (0.5, 1, 5, 10, and 50 mu M) for up to 144 h, we found that 50 mu M triclopyr delayed zebrafish egg hatchability. Yolk sac malabsorption was significant at 0.5, 1, 5, and 10 mu M triclopyr. In zebrafish larvae, uninflated swim bladder was significant only at 50 mu M triclopyr. Furthermore, zebrafish larvae had altered swimming activity after exposure to 10 mu M triclopyr for 144 h. In summary, these comprehensive results indicate that even low triclopyr concentrations can elicit adverse effects during early zebrafish development. (AU)

• FAPESP's process: 18/00229-1 - Cellular and molecular mechanisms of hepatotoxicity and embryotoxicity by emerging contaminants decametilcyclopentasiloxane and triclopyr
• Grantee: Lilian Cristina Pereira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 22/03045-4 - Validation of in vitro cell systems to assess the toxicity of socially relevant environmental pollutants
• Grantee: Lilian Cristina Pereira
• Support Opportunities: Research Grants - Initial Project