| Full text | |
| Author(s): |
Nakamura, Aline Minali
;
Godoy, Andre Schutzer
;
Kadowaki, Marco Antonio Seiki
;
Trentin, Lucas N.
;
Gonzalez, Sinkler E. T.
;
Skaf, Munir S.
;
Polikarpov, Igor
Total Authors: 7
|
| Document type: | Journal article |
| Source: | FEBS Journal; v. 291, n. 22, p. 21-pg., 2024-07-27. |
| Abstract | |
Carboxylesterases comprise a major class of alpha/beta-fold hydrolases responsible for the cleavage and formation of ester bonds. Found ubiquitously in nature, these enzymes are crucial for the metabolism of both endogenous and exogenous carboxyl esters in animals, plants and microorganisms. Beyond their essential physiological roles, carboxylesterases stand out as one of the important classes of biocatalysts for biotechnology. BlEst2, an enzyme previously classified as Bacillus licheniformis esterase, remains largely uncharacterized. In the present study, we elucidate the structural biology, molecular dynamics and biochemical features of BlEst2. Our findings reveal a canonical alpha/beta-hydrolase fold similar to the ESTHER block L of lipases, further augmented by two additional accessory C-terminal domains. Notably, the catalytic domain demonstrates two insertions, which occupy conserved locations in alpha/beta-hydrolase proteins and commonly form the lid domain in lipase structures. Intriguingly, our in vitro cleavage of C-terminal domains revealed the structure of the active form of BlEst2. Upon activation, BlEst2 showed a markedly elevated hydrolytic activity. This observation implies that the intramolecular C-terminal domain serves as a regulatory intramolecular inhibitor. Interestingly, despite exhibiting esterase-like activity, BlEst2 structural characteristics align more closely with lipases. This suggests that BlEst2 could potentially represent a previously unrecognized subgroup within the realm of carboxyl ester hydrolases. (AU) | |
| FAPESP's process: | 19/17373-0 - Computational studies of carbohydrate-active enzymes and cellulose interactions with matrix compounds in plant cell walls |
| Grantee: | Lucas Nascimento Trentin |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 13/08293-7 - CCES - Center for Computational Engineering and Sciences |
| Grantee: | Munir Salomao Skaf |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 21/08780-1 - Enzymatic degradation of microbial biofilm exopolisacharides: structural biophysics, molecular biotechnology and synthetic biochemistry of CAZymes in search for new enzymatic tools for antimicrobial treatments |
| Grantee: | Igor Polikarpov |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 19/17371-8 - Molecular dynamics of enzymes for glycan saccharification |
| Grantee: | Sinkler Eduardo Tormet Gonzalez |
| Support Opportunities: | Scholarships in Brazil - Doctorate |