Combination of Neovestitol and Vestitol Modifies the Profile of Periodontitis-Related Subgingival Multispecies Biofilm

Author(s)

Macedo, Tatiane Tiemi ; Malavazi, Larissa Matias ; Vargas, Gustavo Quilles ; Goncalves, Francisco Jerfeson dos Santos ; Gomes, Aline Paim de Abreu Paulo ; Bueno, Manuela Rocha ; Aguiar da Silva, Lucas Daylor ; Figueiredo, Luciene Cristina ; Bueno-Silva, Bruno

• Document type: Journal article
• Source: BIOMEDICINES; v. 12, n. 6, p. 9-pg., 2024-06-01.

Abstract

The aim of this study was to evaluate the effect of the combination of neovestitol-vestitol (CNV) compounds obtained from Brazilian red propolis on the microbiological profile of a mature multispecies subgingival biofilm. The biofilm with 32 bacterial species associated with periodontitis was formed for seven days using a Calgary device. Treatment with CNV (1600, 800, 400, and 200 mu g/mL), amoxicillin (54 mu g/mL), and vehicle control was performed for 24 h on the last day of biofilm formation. Biofilm metabolic activity and DNA-DNA hybridization (checkerboard) assays were performed. The groups treated with CNV 1600 and amoxicillin reduced 25 and 13 species, respectively, compared to the control vehicle treatment (p <= 0.05); both reduced P. gingivalis, while only CNV reduced T. forsythia. When the data from the two treatments (CNV and AMOXI) were compared, a statistically significant difference was observed in 13 species, particularly members of Socransky's orange complex. Our results showed that CNV at 1600 mu g/mL showed the best results regarding the metabolic activity of mature biofilms and obtained a reduction in species associated with the disease, such as T. forsythia, showing a better reduction than amoxicillin. Therefore, CNV seems to be a promising alternative to eradicate biofilms and reduce their pathogenicity. (AU)

• FAPESP's process: 17/16377-7 - EVALUATION OF THE EFFECT OF PROBIOTIC BACTERIA ON THE IMUNE RESPONSE TO A.ACTINOMYCETEMCOMITANS: A STUDY IN GINGIVAL EPITHELIAL CELLS AND IN EXPERIMENTAL MODEL OF INFECTION IN VIVO.
• Grantee: Manuela Rocha Bueno
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/19691-0 - Bioactive compounds of Brazilian red propolis on periodontal disease
• Grantee: Bruno Bueno Silva
• Support Opportunities: Regular Research Grants