Posttraumatic epilepsy: Integrating clinical, inflammatory, and genetic profiles in traumatic brain injury patients

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Mosini, Amanda C. ; Sanabria, Viviam ; Nakamura, Thabatta K. E. ; Calio, Michele L. ; Pompeu, Clara ; Silva, Clivandir S. ; Nicolicht-Amorim, Priscila ; Naffah-Mazzacoratti, Maria da Graca ; Porcionatto, Marimelia A. ; Mello, Luiz Eugenio ; Foresti, Maira L.

• Document type: Journal article
• Source: Epilepsy Research; v. 205, p. 8-pg., 2024-07-17.

Abstract

Objective: This study aims to assess the clinical, inflammatory, and genetic profiles of traumatic brain injury (TBI) patients over a 2-year follow-up period, focusing on the development of posttraumatic epilepsy (PTE). Methods: Fifty-nine patients with acute TBI were recruited in the emergency unit of a hospital in Brazil. Clinical data and blood samples were collected after 10 days of hospitalization for posterior genetic profile (Apolipoprotein E- ApoE and Glutamic Acid Descarboxylase-GAD sequencing) analyses. A subset of 19 patients were assessed for cytokine markers (mRNA expression). The development of PTE was investigated for two years following TBI. Statistical analyses including univariate analysis, multiple correspondence analysis, and Mann- Whitney test were performed. Results: Analysis revealed an association between severe TBI and requirement for neurosurgery and polytrauma (p<0.05), p <0.05), as well as the development of PTE over a two-year follow-up period (p<0.05). p <0.05). Multiple correspondence analysis identified two distinct profiles associated with PTE and Non-PTE outcomes. The PTE profile showed a higher prevalence of the ApoE genotype E3/E3 and GAD1 SNP (rs769391) genotype AA in our study, while the Non-PTE profile showed a higher presence of E3/E4. mRNA expression analysis demonstrated acute elevated levels of TNF-alpha in the PTE group as compared to Non-PTE patients (6.70+1.53 +1.53 vs 5.31 +0.33, p <0.01). Significance: Our findings underscore the multifactorial nature of aspects potentially contributing to PTE. It is unlikely that any single factor might in isolation have a strong causative influence over the development of epilepsy after TBI. Our results provide a suggestion of potential clustering that might be relevant as prognostic factors for PTE. (AU)

• FAPESP's process: 22/10520-0 - Post-traumatic Epilepsy model induced by lateral fluid percussion in non-human primates
• Grantee: Viviam Sofia Sanabria Calvo
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/24561-5 - Epileptogenesis, biomarkers and post-traumatic epilepsy
• Grantee: Luiz Eugenio Araujo de Moraes Mello
• Support Opportunities: Research Projects - Thematic Grants