| Full text | |
| Author(s): |
de Mello, Diego Claro
;
Menezes, Joice Moraes
;
de Oliveira, Antonio Tarelo Freitas
;
Cristovao, Marcella Maringolo
;
Kimura, Edna Teruko
;
Fuziwara, Cesar Seigi
Total Authors: 6
|
| Document type: | Journal article |
| Source: | ARCHIVES OF ENDOCRINOLOGY METABOLISM; v. 68, p. 13-pg., 2024-01-01. |
| Abstract | |
Modulating the expression of a coding or noncoding gene is a key tool in scientific research. This strategy has evolved methodologically due to advances in cloning approaches, modeling/algorithms in short hairpin RNA (shRNA) design for knockdown efficiency, and biochemical modifications in RNA synthesis, among other developments. Overall, these modifications have improved the ways to either reduce or induce the expression of a given gene with efficiency and facility for implementation in the lab. Allied with that, the existence of various human cell line models for cancer covering different histotypes and biological behaviors, especially for thyroid cancer, has helped improve the understanding of cancer biology. In this review, we cover the most frequently used current techniques for gene modulation in the thyroid cancer field, such as RNA interference (RNAi), short hairpin RNA (shRNA), and gene editing with CRISPR/Cas9 for inhibiting a target gene, and strategies to overexpress a gene, such as plasmid cloning and CRISPRa. Exploring the possibilities for gene modulation allows the improvement of the scientific quality of the studies and the integration of clinicians and basic scientists, leading to better development of translational research. (AU) | |
| FAPESP's process: | 21/01674-1 - Effect of EZH2/PRC2 modulation in agressive thyroid carcinoma |
| Grantee: | Diego Claro de Mello |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 19/17282-5 - Transcriptional and post-transcriptional control in aggressive cancer and metastasis |
| Grantee: | Cesar Seigi Fuziwara |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 19/25116-8 - Modulation of microRNAs and its regulatory target network with therapeutic potential in thyroid cancer: application of gene editing with CRISPR/Cas9 |
| Grantee: | Edna Teruko Kimura |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 20/10403-9 - Transcriptional and post-transcriptional control in aggressive cancer and metastasis |
| Grantee: | Cesar Seigi Fuziwara |
| Support Opportunities: | Scholarships in Brazil - Young Researchers |
| FAPESP's process: | 22/09813-3 - Analysis of EZH2 promoter region in thyroid cancer |
| Grantee: | Marcella Maringolo Cristovão |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 22/03730-9 - Effect of the modulation of EZH2/PRC in lung cancer |
| Grantee: | Joice Moraes Menezes |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 23/14929-3 - Effect of TERT modulation in aggressive thyroid cancer |
| Grantee: | Antonio Tarelo Freitas de Oliveira |
| Support Opportunities: | Scholarships in Brazil - Master |