Cyanidin-3-glucoside reduces cell migration and inflammatory profile of acute leukemia cells

Author(s)

Assaf, Luciene ; Makyiama, Edson Naoto ; Almeida, Beatriz ; de Almeida Junior, Jeremias Martins ; Goncalves, Carlos Eduardo da Silva ; de Freitas, Sumara ; Amon, Rosana Lopes Rodrigues ; Neves, Bruna Roberta Oliveira ; Rogero, Marcelo Macedo ; Fock, Ricardo Ambrosio

• Document type: Journal article
• Source: NATURAL PRODUCT RESEARCH; v. N/A, p. 6-pg., 2025-01-31.

Abstract

Anthocyanins, water-soluble flavonoids found in fruits and vegetables, exhibit diverse biological activities, with cyanidin being the most common pigment. While cyanidin's chemo preventive and antioxidant activity is well-studied, its impact on inflammatory profile and migration capacity of leukemic cells are less understood. This study evaluates the effects of Cyanidin-3-glucoside (C3G) on the inflammatory mechanisms influencing leukemic cell migration. Results show that C3G doses up to 50 mu M do not affect cell metabolism, viability, or cell cycle phases. C3G significantly reduces TNF-alpha, IL-8, CCL2 production, and the p-NF kappa B/NF kappa B ratio in LPS (Lipopolysaccharide)-challenged cells. It also diminishes migration rates in response to LPS or fMLP (N-formyl-methionyl-leucyl-phenylalanine) stimulation and reduces Rho-GTP expression. Thus, C3G modifies the inflammatory and migration properties of leukemic cells, highlighting the potential of anthocyanins as a complementary therapy and an avenue for further therapeutic intervention. (AU)

• FAPESP's process: 21/08658-1 - Influence of protein malnutrition in the elderly on the immunomodulatory aspects of mesenchymal stromal cells and their action in the regulation of hematopoiesis
• Grantee: Ricardo Ambrósio Fock
• Support Opportunities: Regular Research Grants