In vitro reduction of enamel erosion by sugarcane-derived cystatin associated with sodium trimetaphosphate

Author(s)

Ferrari, Carolina Ruis ; Kitamoto, Karolyne Sayuri de Araujo ; Pela, Vinicius Taioqui ; Taira, even Akemi ; Araujo, Tamara Teodoro ; Thomassian, Larissa Tercilia Grizzo ; Henrique-silva, Flavio ; Pessan, Juliano Pelim ; Buzalaf, Marilia Afonso Rabelo

• Document type: Journal article
• Source: BRAZILIAN ORAL RESEARCH; v. 38, p. 10-pg., 2024-01-01.

Abstract

The objective of this in vitro study was to assess the efficacy of CaneCPI-5, either alone or in combination with various concentrations of sodium trimetaphosphate (TMP) in protecting against initial enamel erosion. A total of 135 bovine enamel specimens were prepared and categorized into nine groups (n/group=15) according to the following treatments: Deionized water; Commercial solution 1.0% TMP; 3.0% TMP; 0.1 mg/mL CaneCPI-5+0.5% TMP; 0.1 mg/mL CaneCPI-5+1.0%TMP; and 0.1 mg/mL CaneCPI-5+3.0%TMP. The specimens were treated with the respective solutions for 2 h, followed by acquired enamel pellicle formation for 2 h and exposure to 0.65% citric acid (CA) for 1 min. These procedures were repeated once a day for three consecutive days. Demineralization was assessed by the percentage change in surface hardness (%CSH) and calcium release into CA, analyzed by the Arsenazo III method. The data were evaluated using Kruskal-Wallis/Dunn's tests. Regarding %CSH, CaneCPI-5+3.0%TMP was the most effective treatment when compared to the CaneCPI-5 group alone. As for calcium release into CA, the CaneCPI-5+0.5% TMP and CaneCPI-5 groups (both with lower calcium release) did not significantly differ from the commercial solution. In conclusion, combination of CaneCPI-5 with TMP enhances the protective potential against initial enamel erosion in vitro. (AU)

• FAPESP's process: 22/11297-3 - Acquired pellicle engineering for the control of dental erosion: in vitro evaluation of the protective potential of different concentrations of sodium trimetaphosphate associated or not with CaneCPI-5
• Grantee: Karolyne Sayuri de Araujo Kitamoto
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 21/14161-2 - Recombinant production and functional evaluation of a hybrid recombinant protein with potential to interact with hydroxyapatite to protect against dental caries and erosion
• Grantee: Carolina Ruis Ferrari
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)