| Full text | |
| Author(s): |
Oshiquiri, Leticia Harumi
;
Pereira, Lucas Matheus Soares
;
Maues, David Batista
;
Milani, Elizabete Rosa
;
Silva, Alinne Costa
;
Jesus, Luiz Felipe de Morais Costa de
;
Silva-Neto, Julio Alves
;
Veras, Flavio Protasio
;
de Paula, Renato Graciano
;
Silva, Roberto Nascimento
Total Authors: 10
|
| Document type: | Journal article |
| Source: | JOURNAL OF FUNGI; v. 10, n. 12, p. 25-pg., 2024-12-01. |
| Abstract | |
Recent research has revealed the calcium signaling significance in the production of cellulases in Trichoderma reesei. While vacuoles serve as the primary calcium storage within cells, the function of vacuolar calcium transporter proteins in this process remains unclear. In this study, we conducted a functional characterization of four vacuolar calcium transport proteins in T. reesei. This was accomplished by the construction of the four mutant strains triangle trpmc1, triangle tryvc1, triangle tryvc3, and triangle tryvc4. These mutants displayed enhanced growth when subjected to arabinose, xylitol, and xylose. Furthermore, the mutants triangle trpmc1, triangle tryvc1, and triangle tryvc4 showed a reduction in growth under conditions of 100 mM MnCl2, implying their role in manganese resistance. Our enzymatic activity assays revealed a lack of the expected augmentation in cellulolytic activity that is typically seen in the parental strain following the introduction of calcium. This was mirrored in the expression patterns of the cellulase genes. The vacuolar calcium transport genes were also found to play a role in the expression of genes involved with the biosynthesis of secondary metabolites. In summary, our research highlights the crucial role of the vacuolar calcium transporters and, therefore, of the calcium signaling in orchestrating cellulase and hemicellulase expression, sugar utilization, and stress resistance in T. reesei. (AU) | |
| FAPESP's process: | 19/19569-0 - Functional characterization of the calcium-dependent signaling pathway in the regulation of cellulases expression by the fungus Trichoderma reesei |
| Grantee: | Letícia Harumi Oshiquiri |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 19/11655-4 - Functional studies of gene regulatory networks in Trichoderma reesei during the cellulases formation |
| Grantee: | Roberto do Nascimento Silva |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 16/23233-9 - Characterization of calcium-dependent signaling pathway in the secretion and cellulase expression by Trichoderma reesei (Hypocrea jecorina |
| Grantee: | Renato Graciano de Paula |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |