| Full text | |
| Author(s): Show less - |
Kim, Young Chan
;
Watanabe, Yasunori
;
Arlen-Celina, Luecke
;
Song, Xiyong
;
Souza, Raquel de Oliveira
;
Stass, Robert
;
Azar, Sasha R.
;
Rossi, Shannan L.
;
Claser, Carla
;
Kuemmerer, Beate Mareike
;
Crispin, Max
;
Bowden, Thomas A.
;
Huiskonen, Juha T.
;
Reyes-Sandoval, Arturo
Total Authors: 14
|
| Document type: | Journal article |
| Source: | NPJ VACCINES; v. 9, n. 1, p. 13-pg., 2024-12-17. |
| Abstract | |
Virus-like particles (VLPs) are an established vaccine platform and can be strong immunogens capable of eliciting both humoral and cellular immune responses against a range of pathogens. Here, we show by cryo-electron microscopy that VLPs of Mayaro virus, which contain envelope glycoproteins E1-E2 and capsid, exhibit an architecture that closely resembles native virus. In contrast to monomeric and soluble envelope 2 (E2) glycoprotein, both VLPs as well as the adenovirus and modified vaccinia virus Ankara (MVA) vaccine platforms expressing the equivalent envelope glycoproteins E1-E2, and capsid induced highly neutralising antibodies after immunisation. The levels of neutralising antibodies elicited by the viral-vectored vaccines of structural proteins and VLPs increased significantly upon boosting. Immunisation of Mayaro virus VLPs in mice with or without an adjuvant (poly:IC) yielded similar levels of neutralising antibodies suggesting that the VLPs may be used for immunisation without the need for an adjuvant. A single or two doses of non-adjuvanted 5 mu g of MAYV VLP vaccination provided significant protection against viremia and MAYV-induced foot swelling in the C57BL/6 mouse challenge model. MAYV VLPs represent a non-infectious vaccine candidate, which may constitute a complementary option for future immunisation strategies against this important emerging alphavirus. (AU) | |
| FAPESP's process: | 22/15124-6 - Study of humoral and cellular immune response modulation induced by Mayaro and Chikungunya viruses in coinfected mice |
| Grantee: | Raquel de Oliveira Souza |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 18/24470-0 - Study of immune-pathological alterations induced during co-infection with Alpha virus and Malaria in murine model |
| Grantee: | Carla Claser |
| Support Opportunities: | Research Grants - Young Investigators Grants |