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Arboviruses VLP production in insect cells.

Grant number: 16/22780-6
Support Opportunities:Regular Research Grants
Duration: April 01, 2017 - June 30, 2019
Field of knowledge:Biological Sciences - Biology
Principal Investigator:Soraia Attie Calil Jorge
Grantee:Soraia Attie Calil Jorge
Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Arboviruses are viruses transmitted by mosquitoes and other arthropods. In Brazil, there are 210 different types of arboviruses, among these arboviruses, largely belongs to the families of Togaviridade and Flaviviridae, Alphavirus and Flavivirus genera, respectively. Some arboviruses are responsible for neglected disease. In this sense, our research group has traditionally worked with the theme of neglected viral diseases, while developing different recombinant expression systems. We could express the rabies glycoprotein in Drosophila melanogaster S2 cells and thus our group acquired excellence in working with insect cells, and national reference in this regard (FAPESP 2002 / 09482-3). We developed in our Institute the Semliki Forest Virus expression system, like a very promising recombinant alphavirus.The striking lack of scientific data on the molecular biology of many of arboviruses and particularly the shortages of papers reporting the expression of these proteins in recombinant systems make this challenging proposition. At the same time, the results of the research proposed here should represent an important step in the understanding of these viruses and their infection, and especially for a future proposition vaccine preparation needed in various locations in Latin America and of strategic importance when analyzing the recent urban cases of arboviruses and viral tropism in different vectors.In this way, our goal in this project is to establish methodology for viral like particles (VLPs) production and / or recombinant proteins Mayaro virus (MAYV), Chikungunya (CHIKV) and Zika (ZIKV). (AU)

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE MELLO, RENATA GOIS; BERNARDINO, THAISSA CONSONI; GUARDALINI, LUIS GIOVANI OLIVEIRA; ASTRAY, RENATO MANCINI; ANTONIAZZI, MARTA MARIA; JARED, SIMONE GONCALVES SILVA; NUNEZ, EUTIMIO GUSTAVO FERNANDEZ; JORGE, SORAIA ATTIE CALIL. Zika virus-like particles (VLPs) produced in insect cells. FRONTIERS IN PHARMACOLOGY, v. 14, p. 9-pg., . (16/22780-6)
GUARDALINI, LUIS GIOVANI OLIVEIRA; DIAS, VINICIUS ARAGAO TEJO; LEME, JACI; BERNARDINO, THAISSA CONSONI; ASTRAY, RENATO MANCINI; DA SILVEIRA, SUELLEN REGINA; HO, PAULO LEE; TONSO, ALDO; JORGE, SORAIA ATTIE CALIL; NUNEZ, EUTIMIO GUSTAVO FERNANDEZ. Comparison of chemometric models using Raman spectroscopy for offline biochemical monitoring throughout the VLP-making upstream process. Biochemical Engineering Journal, v. 198, p. 12-pg., . (18/10538-1, 22/02713-3, 22/04711-8, 16/22780-6)
OLIVEIRA GUARDALINI, LUIS GIOVANI; DA SILVA CAVALCANTE, PAULO EDUARDO; LEME, JACI; DE MELLO, RENATA GOIS; BERNARDINO, THAISSA CONSONI; ASTRAY, RENATO MANCINI; TONSO, ALDO; CALIL JORGE, SORAIA ATTIE; FERNANDEZ NUNEZ, EUTIMIO GUSTAVO. Oxygen uptake and transfer rates throughout production of recombinant baculovirus and rabies virus-like particles. Process Biochemistry, v. 124, p. 12-pg., . (18/10538-1, 16/22780-6)
GUARDALINI, LUIS GIOVANI OLIVEIRA; LEME, JACI; CAVALCANTE, PAULO EDUARDO DA SILVA; DE MELLO, RENATA GOIS; BERNARDINO, THAISSA CONSONI; JARED, SIMONE GONCALVES SILVA; ANTONIAZZI, MARTA MARIA; ASTRAY, RENATO MANCINI; TONSO, ALDO; NUNEZ, EUTIMIO GUSTAVO FERNANDEZ; et al. Sf9 Cell Metabolism Throughout the Recombinant Baculovirus and Rabies Virus-Like Particles Production in Two Culture Systems. MOLECULAR BIOTECHNOLOGY, v. N/A, p. 11-pg., . (18/10538-1, 16/22780-6)
GUARDALINI, LUIS GIOVANI OLIVEIRA; CAVALCANTE, PAULO EDUARDO DA SILVA; LEME, JACI; DE MELLO, RENATA GOIS; BERNARDINO, THAISSA CONSONI; ASTRAY, RENATO MANCINI; BARBOSA, EDUARDO; DA SILVEIRA, SUELLEN REGINA; HO, PAULO LEE; TONSO, ALDO; et al. Biochemical monitoring throughout all stages of rabies virus-like particles production by Raman spectroscopy using global models. Journal of Biotechnology, v. 363, p. 13-pg., . (16/22780-6, 18/10538-1)
LEME, JACI; OLIVEIRA GUARDALINI, LUIS GIOVANI; BERNARDINO, THAISSA CONSONI; ASTRAY, RENATO MANCINI; TONSO, ALDO; FERNANDEZ NUNEZ, EUTIMIO GUSTAVO; CALIL JORGE, SORAIA ATTIE. Sf9 Cells Metabolism and Viability When Coinfected with Two Monocistronic Baculoviruses to Produce Rabies Virus-like Particles. MOLECULAR BIOTECHNOLOGY, v. N/A, p. 13-pg., . (16/22780-6, 18/10538-1)

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