Induced-pluripotent stem cells and neuroproteomics as tools for studying neurodegeneration

Author(s)

Brandao-Teles, Caroline ; Zuccoli, Giuliana S. ; Vrechi, Talita Aparecida de Moraes ; Ramos-da-Silva, Livia ; Santos, Aline Valeria Sousa ; Crunfli, Fernanda ; Martins-de-Souza, Daniel

• Document type: Journal article
• Source: BIOCHEMICAL SOCIETY TRANSACTIONS; v. 52, n. 1, p. 14-pg., 2024-01-30.

Abstract

The investigation of neurodegenerative diseases advanced significantly with the advent of cell-reprogramming technology, leading to the creation of new models of human illness. These models, derived from induced pluripotent stem cells (iPSCs), facilitate the study of sporadic as well as hereditary diseases and provide a comprehensive understanding of the molecular mechanisms involved with neurodegeneration. Through proteomics, a quantitative tool capable of identifying thousands of proteins from small sample volumes, researchers have attempted to identify disease mechanisms by detecting differentially expressed proteins and proteoforms in disease models, biofluids, and postmortem brain tissue. The integration of these two technologies allows for the identification of novel pathological targets within the realm of neurodegenerative diseases. Here, we highlight studies from the past 5 years on the contributions of iPSCs within neuroproteomic investigations, which uncover the molecular mechanisms behind these illnesses. (AU)

• FAPESP's process: 19/22398-2 - The role of cholesterol synthesis in the mode of action of clozapine in schizophrenia models
• Grantee: Fernanda Crunfli
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/25588-1 - From the basic understanding to clinical biomarkers to schizophrenia: a neuroproteomics-centered multidisciplinary study
• Grantee: Daniel Martins-de-Souza
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/14666-4 - Influence of the endocannabinoid system on the energetic profile of neural cells during neurodevelopment: implications in Schizophrenia
• Grantee: Giuliana da Silva Zuccoli
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 22/10140-3 - The role of 14-3-3 proteins in autophagy in the context of Schizophrenia
• Grantee: Talita Aparecida de Moraes Vrechi
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 22/12693-0 - Neural cell culture
• Grantee: Caroline Brandão Teles Rodrigues
• Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training