Simplifying traditional approaches for accessible analysis of snake venom enzymes

Author(s)

Wiezel, Gisele Adriano ; Oliveira, Isadora Sousa de ; Arantes, Eliane Candiani

• Document type: Journal article
• Source: Toxicon; v. 255, p. 5-pg., 2025-01-22.

Abstract

Snake venoms enzymes affect diverse physiological mechanisms leading to effects such as inflammation, edema, hemolysis, and blood clotting disorders. In this report, we describe modifications to classical assays for assessing the enzymatic activity of snake venom phospholipase A2 (PLA2) and phosphodiesterase (PDE), including the adaptation of the PDE assay to an agar plate. A final staining step, using Stains-all (R), was added to the PLA2 activity assay on an egg yolk-containing agar plate. Moreover, PDE activity was successfully and qualitative assed using an agar plate-immobilized bis- p-nitrophenyl-phosphate. The modified methods introduced in this study improve accessibility for a broader spectrum of researchers, enabling venom-related investigations in any laboratory setting, with special relevance for regions where snakebites are most prevalent. (AU)

• FAPESP's process: 20/13176-3 - Human monoclonal antibodies (scFv) discovery with cross-reactivity and pH-dependent to metalloproteases from Bothrops spp
• Grantee: Isadora Sousa de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/00586-6 - Characterization of a phospholipase A2 inhibitor from the Crotalus durissus terrificus venom gland: a possible adjuvant in the antivenom therapy
• Grantee: Gisele Adriano Wiezel
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 21/11936-3 - Center for Translational Science and Biopharmaceutical Development
• Grantee: Benedito Barraviera
• Support Opportunities: Research Grants - Science Centers for Development
• FAPESP's process: 19/10173-6 - Production, modification and characterization of animal toxins with potential biotechnological application
• Grantee: Eliane Candiani Arantes Braga
• Support Opportunities: Regular Research Grants