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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

An MLSA-based online scheme for the rapid identification of Stenotrophomonas isolates

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Author(s):
Ramos, Patricia Locosque [1, 2] ; Moreira-Filho, Carlos Alberto [1, 2] ; Van Trappen, Stefanie [3] ; Swings, Jean [3] ; De Vos, Paul [3] ; Barbosa, Heloiza Ramos [4] ; Thompson, Cristiane Carneiro [5] ; Ribeiro Vasconcelos, Ana Tereza [6] ; Thompson, Fabiano Lopes [5]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Ctr Pesquisas Biotecnol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med, Dept Pediat, BR-01246903 Sao Paulo - Brazil
[3] Univ Ghent, Microbiol Lab, B-9000 Ghent - Belgium
[4] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508 Sao Paulo - Brazil
[5] Univ Fed Rio de Janeiro, Inst Biol, Dept Genet, Rio De Janeiro - Brazil
[6] Lab Nacl Comp Cient, Dept Matemat Aplicada & Computac, Petropolis, RJ - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Memórias do Instituto Oswaldo Cruz; v. 106, n. 4, p. 394-399, JUN 2011.
Web of Science Citations: 7
Abstract

An online scheme to assign Stenotrophomonas isolates to genomic groups was developed using the multilocus sequence analysis (MLSA), which is based on the DNA sequencing of selected fragments of the housekeeping genes ATP synthase alpha subunit (atpA), the recombination repair protein (recA), the RNA polymerase alpha subunit (rpoA) and the excision repair beta subunit (uvrB). This MLSA-based scheme was validated using eight of the 10 Stenotrophomonas species that have been previously described. The environmental and nosocomial Stenotrophomonas strains were characterised using MLSA, 16S rRNA sequencing and DNA-DNA hybridisation (DDH) analyses. Strains of the same species were found to have greater than 95% concatenated sequence similarity and specific strains formed cohesive readily recognisable phylogenetic groups. Therefore, MLSA appeared to be an effective alternative methodology to amplified fragment length polymorphism fingerprint and DDH techniques. Strains of Stenotrophomonas can be readily assigned through the open database resource that was developed in the current study (www.steno.lncc.br/). (AU)

FAPESP's process: 04/00814-9 - Biodiversity, comparative genomics and evolution in the family Vibrionaceae (bacteria)
Grantee:Fabiano Lopes Thompson
Support Opportunities: Research Grants - Young Investigators Grants