Amine-Polyether-Epoxide Nanoplatform-Driven Seed Germination, Plant Growth, and Nutrient Uptake for Sustainable Agriculture

Author(s):Show less -

Fico, Bruno A. ; Andrada, Heber E. ; Alves, Felipe B. ; da Silva, Enzo E. ; Reinaldi, Julia S. ; Tavares, Denise C. ; Squarisi, Iara S. ; Nuevo, Laura G. ; Montanha, Gabriel Sgarbiero ; de Carvalho, Hudson W. P. ; Chavez, Fabian Vaca ; Molina, Eduardo F.

• Document type: Journal article
• Source: ACS OMEGA; v. 10, n. 12, p. 14-pg., 2025-03-20.

Abstract

Polymeric systems can facilitate the diffusion of micronutrients through seeds, offering an innovative and sustainable way to improve plant health and increase food production. In the present work, a polymeric nanogel based on polyether-POE-diamine and bisepoxide was synthesized and in-depth characterized, encompassing its morphological characteristics (by Transmission Electron Microscopy, TEM), the size distribution, and surface charge of the particles (by dynamic light scattering, DLS and zeta potential, zeta). The formation of the polymeric network was assessed using Fourier-transform infrared spectroscopy (FTIR) and proton nuclear magnetic resonance (1H NMR), confirming the opening of the epoxide ring and the formation of amine and glycol groups. The effects of seed priming with the nanogel (well-defined spherical particles, with sizes around 120 nm, named POE-gel) on the early growth stage of cucumber plants (Cucumis sativus) exhibited a discernible ameliorative impact on root and shoot lengths, with average improvements of 33% and 90%, respectively, compared to the control group after 12 days. Extensive investigation using germination assays and micro X-ray fluorescence (mu-XRF) analysis indicated potential applications of the POE-gel as a carrier for micronutrients (such as Fe3+). The seeds treated with the iron-loaded POE-gel presented a substantial positive effect on root length, exhibiting a 3-fold increase in size compared with the control-Fe treatment at the same concentration. The loaded POE-gel effectively penetrated through the seed compartments, providing an even distribution of iron ions and facilitating the uptake of nutrients (K, Mn, and Zn) by the seeds. Toxicological assays using zebrafish (Danio rerio), seeds, and leaves revealed notable safety of the iron-loaded and unloaded POE-gel for agricultural purposes. Employing water as the only solvent in the synthesis, as well as eliminating the use of a catalyst, makes this class of polymeric particles suitable for sustainable agricultural applications. The findings of this work contribute to the development of sustainable agriculture, presenting an innovative approach to enhancing plant development and nutrient uptake through the application of polymeric nanogels as a seed priming technology. (AU)

• FAPESP's process: 21/06552-1 - TASTYGEL: sustainable release of nutrients by smart micro- and nanogels based on polyetheramines-bisepoxide for Agriculture purposes
• Grantee: Eduardo Ferreira Molina
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 22/13408-7 - Micro- and nanogel based on polyetheramine-bisepoxide as new carrier for controlled release of plant micronutrients
• Grantee: Felipe Breda Alves
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 21/14619-9 - Multi-user Equipment approved in grant 2021/06552-1: equipment DLS/zeta potential
• Grantee: Eduardo Ferreira Molina
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 22/06507-9 - Micronutrient simultaneous release from nanogels based on polyetheramines and bisepoxide
• Grantee: Heber Eduardo Andrada
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 20/07721-9 - Time-resolved element mapping and characterization of storage proteins during soybean seeds development: a bottom-up strategy to increase protein content and quality
• Grantee: Gabriel Sgarbiero Montanha
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)