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Molecular and biological characterization of tobacco mild green mosaic virus from Petunia (Petunia x hybrida) in Brazil

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Author(s):
Bello, Vinicius Henrique ; Pires, Lorenna Leal ; Pires, Gleice Rafaela Renunza ; Bernardi, Gustavo Verruma ; Ramos-Gonzalez, Pedro Luis ; Salaroli, Renato Barbosa ; Kitajima, Elliot Watanabe
Total Authors: 7
Document type: Journal article
Source: CIENTIA AGRICOL; v. 82, p. 11-pg., 2025-01-01.
Abstract

An isolate of the tobamovirus tobacco mild green mosaic virus (TMGMV), Tobamovirus mititessellati, was found naturally infecting petunia (Petunia x hybrida) cv. Pinstripe, causing reduced leaf size, mottling, and color breaking of flowers, in an experimental field at Piracicaba, S & atilde;o Paulo state, Brazil. TMGMV was identified by virion morphology, cytopathology, transmission experiments, and molecular assays. The entire nucleotide sequence of this petunia isolate of TMGMV (TMGMV-Pet) was obtained and demonstrated, in essence, to be similar to previously analyzed isolates. One hundred and twenty plant species were assayed by mechanical transmission, and 35 of them from nine families, both previously reported and unreported species, were shown to be experimentally susceptible to TMGMV-Pet. On the other hand, certain species, among 85 unsusceptible hosts for TMGMV-Pet, known to be susceptible to TMGMV, failed to be infected. Cytopathological studies on naturally and experimentally infected host plants revealed that virions of TMGMV-Pet produced para-crystalline aggregates, but not angular arrays, which are considered characteristic of TMGMV. As previously described, thin sections revealed the presence of pseudo-virions in the stroma of chloroplasts in TMGMV-Pet-infected tobacco (Nicotiana tabacum), and, additionally, in other hosts such as N. clevelandii, N. megalosyphon, and petunia indicating that this process is not restricted to tobacco. (AU)

FAPESP's process: 21/02179-4 - Evaluation of the diversity of molecular pathogens (virus and viroids) of plants in Brazi: Phase III - 2021/2023
Grantee:Elliot Watanabe Kitajima
Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
FAPESP's process: 20/05563-7 - The amplifier host hypothesis in the epidemiology of the Golden Mosaic of tomatoes, caused by Tomato Severe Rugose Virus (ToSRV): experimental proof and determination of the conditions of occurrence
Grantee:Vinicius Henrique Bello
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 22/05043-9 - Solanum violifolium as a natural host for three different viruses transmitted by Brevipalpus mites: a model for the study of the interaction among these viruses
Grantee:Gustavo Verruma Bernardi
Support Opportunities: Scholarships in Brazil - Scientific Initiation