Impact of citric acid and conventional denture cleansers on surface properties and antimicrobial performance of conventionally heat-processed acrylic resin: An in vitro study

Author(s)

Malheiros, Samuel Santana ; Celles, Cicero Andrade Sigiliao ; Borges, Maria Helena Rossy ; Correa, Mirtes Maria Ferreira ; de Andrade, Catia Sufia Alves Freire ; Neto, Joao Vicente Calazans ; Barao, Valentim Adelino Ricardo ; Nagay, Bruna Egumi

• Document type: Journal article
• Source: JOURNAL OF PROSTHETIC DENTISTRY; v. 133, n. 6, p. 9-pg., 2025-06-01.

Abstract

Statement of problem. Effective decontamination of denture base acrylic resin is essential for biofilm removal but can compromise the integrity of the material. Citric acid has shown promise as a potent antimicrobial agent, though its effectiveness as a denture cleanser is unknown. Purpose. The purpose of this in vitro study was to evaluate the effect of citric acid on the surface properties and efficacy of biofilm removal on denture base acrylic resin compared with commonly used denture cleansers. Material and methods. A total of 130 conventional, heat-processed denture base acrylic resin disks were randomly allocated into 1 of 5 groups based on the decontamination solution applied: control 0.9% sodium chloride (NaCl), 0.1% sodium hypochlorite (SH), Periogard (chlorhexidine digluconate), effervescent denture cleansing tablets (Corega Tabs), and 10% citric acid (CA). Surface properties (roughness, hydrophilicity, microhardness, and color stability) were assessed at 3 time points: baseline (T0), after 5 minutes of exposure (T1), and after 1 week of exposure (T2) to the respective solution. A microcosm biofilm was formed on specimen surfaces to evaluate antimicrobial efficacy of the decontamination solution groups, through assessment of microbial viability, metabolic activity, biofilm dry weight, and morphology. A 2-way repeated measures ANOVA was performed for surface properties evaluations and for microbiological analysis, 1-way ANOVA was used (alpha=.05). Results. No significant differences in average surface roughness (Ra) were observed between T0, T1, and T2 across all groups (P>.05). CA significantly increased surface hydrophilicity at T2 (P<.001) compared with the other groups. No significant differences in Delta E00 were seen among the groups: all values near the 50% perceptibility threshold. Compared with the control group, all denture cleansers significantly reduced the viability of total microorganisms and Candida species, with no viable colonies detected after treatment (P<.001). However, CA demonstrated a robust antimicrobial effect, with a significant reduction in biofilm dry weight and metabolic activity (P<.001), suggesting superior biofilm disruption compared with the other groups. Conclusions. Citric acid effectively increased hydrophilicity of conventional, heat-processed acrylic resin without negatively impacting surface roughness, microhardness, or color stability. (J Prosthet Dent 2025;133:1585.e1-e9) (AU)

• FAPESP's process: 22/15677-5 - Synthesis of a bilayer coating functionalized with zinc and photoactivated chitosan-antibiotic complex for controlled drug release in dental implants
• Grantee: Samuel Santana Malheiros
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 22/16267-5 - Antimicrobial and osteogenic surface development by electrodeposition of polypyrrole film on titanium surfaces treated by plasma electrolytic oxidation: a multifunctional coating for dental implants
• Grantee: Valentim Adelino Ricardo Barão
• Support Opportunities: Regular Research Grants