| Full text | |
| Author(s): |
Santos, Nilton J.
;
Mosele, Francielle C.
;
Barquilha, Caroline N.
;
Barbosa, Isabela C.
;
Lima, Flavio de Oliveira
;
Barbosa, Guilherme Oliveira
;
Carvalho, Hernandes F.
;
Delella, Flavia Karina
;
Felisbino, Sergio Luis
Total Authors: 9
|
| Document type: | Journal article |
| Source: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 26, n. 11, p. 13-pg., 2025-05-30. |
| Abstract | |
Prostate cancer (PCa) is the second most common cancer and the second leading cause of cancer-related mortality among men. Gene expression analysis has been crucial in understanding tumor biology and providing disease progression markers. Cell surface glycoproteins and those in the extracellular matrix play significant roles in the PCa microenvironment by promoting migration, invasion, and metastasis. The molecular and histopathological heterogeneity of prostate tumors necessitates a new marker discovery to better stratify patients at risk for poor prognosis. In this study, our objectives were to investigate and characterize the localization and expression of SLIT/ROBO in PCa samples from transgenic mice and human tumor samples, aiming to identify novel prognostic markers and potential therapeutic targets. We conducted histopathological, immunohistochemical, and bioinformatics analyses on prostate tumors from two knockout mice models (Pb-Cre4/Ptenf/f and Pb-Cre4/Trp53f/f;Rb1f/f) and human prostate tumors. Transcriptomic analyses revealed special changes in the expression of genes related to the SLIT/ROBO neural signaling pathway. We further characterized the gene and protein expression of the SLIT/ROBO pathway in knockout animal samples, and protein expression in the PCa samples of patients with different Gleason scores. Public datasets with clinical data from patients (The Human Protein Atlas, cBioPortal, SurvExpress and CamcAPP) were used to validate the gene and protein expression of SLIT1, SLIT2, ROBO1, and ROBO4, correlating these alterations with the prognosis of subgroups of patients. Our findings highlight potential biomarkers of the SLIT/ROBO pathway with prognostic and predictive value, as well as promising therapeutic targets for PCa. (AU) | |
| FAPESP's process: | 19/19644-1 - Prostate cancer: involvement of adiponectin and type X collagen pathways |
| Grantee: | Sérgio Luis Felisbino |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 21/02303-7 - Single cell analyses and non-linear optics approaches of prostate biology |
| Grantee: | Hernandes Faustino de Carvalho |
| Support Opportunities: | Research Projects - Thematic Grants |