Intrasession, intersession, and interexaminer variabilities of retinal nerve fiber layer measurements with spectral-domain OCT

Author(s)

Cremasco, Fernanda ^[1] ; Massa, Graziela ^[1] ; Vidotti, Vanessa Goncalves ^[1] ; de Carvalho Lupinacci, Alvaro Pedroso ^[1] ; Costa, Vital Paulino ^[1]

Affiliation

^[1] Univ Estadual Campinas, Dept Ophthalmol, Glaucoma Serv, Campinas - Brazil

• Document type: Journal article
• Source: EUROPEAN JOURNAL OF OPHTHALMOLOGY; v. 21, n. 3, p. 264-270, MAY-JUN 2011.
• Web of Science Citations: 10

Abstract

Purpose. To evaluate the intrasession, intersession, and interexaminer variabilities of retinal nerve fiber layer measurements (RNFL) with spectral-domain optical coherence tomography (OCT). Methods. A total of 32 healthy individuals and 34 patients with chronic glaucoma underwent RNFL measurements with the Cirrus HD-OCT Model 4000 (Carl Zeiss Meditec, Dublin, CA, USA) 5 times during the same sitting by one examiner to assess intrasession variability. The same examiner performed RNFL measurements in the same patients on 5 different days to assess intersession variability. A second examiner performed RNFL measurements in the same patients to assess interexaminer variability. The coefficients of variation and intraclass correlation coefficients were obtained for the following parameters: average thickness, quadrant thickness, and Clock hour thickness measurements. Results. Intrasession variability: In patients with glaucoma, coefficients of variation ranged from 4.51% to 11.84%. Intraclass correlation coefficients ranged from 0.74 to 0.99. In healthy individuals, coefficients of variation ranged from 2.92% to 6.99%. Intraclass correlation coefficients ranged from 0.89 to 0.98. Intersession variability: In patients with glaucoma, coefficients of variation ranged from 3.68% to 10.50%. Intraclass correlation coefficients ranged from 0.82 to 0.99. In healthy individuals, coefficients of variation ranged from 3.13% to 6.92%. Intraclass correlation coefficients ranged from 0.87 to 0.99. Interexaminer variability: In patients with glaucoma, coefficients of variation ranged from 2.62% to 14.94%. Intraclass correlation coefficients ranged from 0.55 to 0.98. In healthy individuals, coefficients of variation ranged from 2.04% to 7.31%. Intraclass correlation coefficients ranged from 0.86 to 0.98. Conclusions. These findings indicate that RNFL measurements with spectral-domain OCT display excellent reproducibility, with low intrasession, intersession, and interexaminer variabilities. (AU)

• FAPESP's process: 07/51281-9 - Early glaucoma diagnosis and progression analysis based on machine learning hybrid classifiers
• Grantee: Vital Paulino Costa
• Support Opportunities: Regular Research Grants