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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The CEBPA gene is down-regulated in acute promyelocytic leukemia and its upstream promoter, but not the core promoter, is highly methylated

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Author(s):
Santana-Lemos, Barbara Amelia [1] ; Alencar de Lima Lange, Ana Paula [1] ; de Lira Benicio, Mariana Tereza [1] ; da Silva Jose, Thiago Donizete [1] ; Lucena-Araujo, Antonio Roberto [1] ; Krause, Alexandre [1] ; Thome, Carolina Hassibe [1] ; Rego, Eduardo Magalhaes [2]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Med Sch Ribeirao Preto, Natl Inst Sci & Technol Stem Cell & Cell Therapy, Dept Internal Med, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Med Sch Ribeirao Preto, Natl Inst Sci & Technol Stem Cell & Cell Therapy, Div Hematol Oncol, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Haematologica; v. 96, n. 4, p. 617-620, APR 2011.
Web of Science Citations: 10
Abstract

impairment of CCAAT Enhancer Binding Protein alpha (CEBPA) function is a common finding in acute myeloid leukemia; nevertheless, its relevance for acute promyelocytic leukemia pathogenesis is unclear. We analyzed the expression and assessed the methylation status of the core and upstream promoters of CEBPA in acute promyelocytic leukemia at diagnosis. Patients with acute promyelocytic leukemia (n=18) presented lower levels of CEBPA expression compared to healthy controls (n=5), but higher levels than those in acute myeloid leukemia with t(8;21) (n=9) and with inv(16) (n=5). Regarding the core promoter, we detected no methylation in 39 acute promyelocytic leukemia samples or in 8 samples from controls. In contrast, analysis of the upstream promoter showed methylation in 37 of 39 samples, with 17 patients showing methylation levels over 30%. Our results corroborate data obtained in animal models showing that CEBPA is down-regulated in acute promyelocytic leukemia stem cells and suggest that epigenetic mechanisms may be involved. (AU)