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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Copia Retrotransposon in the Zaprionus Genus: Another Case of Transposable Element Sharing with the Drosophila melanogaster Subgroup

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Author(s):
de Setta, Nathalia [1, 2] ; Van Sluys, Marie-Anne [1] ; Capy, Pierre [3, 4] ; Aparecida Carareto, Claudia Marcia [2]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Dept Bot, Genom & Transposable Elements Lab, BR-05508090 Sao Paulo - Brazil
[2] Sao Paulo State Univ, UNESP, Dept Biol, Lab Mol Evolut, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[3] CNRS, UPR9034, Lab Evolut Genomes & Speciat, F-91198 Gif Sur Yvette - France
[4] Univ Paris 11, F-91405 Orsay - France
Total Affiliations: 4
Document type: Journal article
Source: Journal of Molecular Evolution; v. 72, n. 3, p. 326-338, MAR 2011.
Web of Science Citations: 4
Abstract

Copia is a retrotransposon that appears to be distributed widely among the Drosophilidae subfamily. Evolutionary analyses of regulatory regions have indicated that the Copia retrotransposon evolved through both positive and purifying selection, and that horizontal transfer (HT) could also explain its patchy distribution of the among the subfamilies of the melanogaster subgroup. Additionally, Copia elements could also have transferred between melanogaster subgroup and other species of Drosophilidae-D. willistoni and Z. tuberculatus. In this study, we surveyed seven species of the Zaprionus genus by sequencing the LTR-ULR and reverse transcriptase regions, and by using RT-PCR in order to understand the distribution and evolutionary history of Copia in the Zaprionus genus. The Copia element was detected, and was transcriptionally active, in all species investigated. Structural and selection analysis revealed Zaprionus elements to be closely related to the most ancient subfamily of the melanogaster subgroup, and they seem to be evolving mainly under relaxed purifying selection. Taken together, these results allowed us to classify the Zaprionus sequences as a new subfamily-ZapCopia, a member of the Copia retrotransposon family of the melanogaster subgroup. These findings indicate that the Copia retrotransposon is an ancient component of the genomes of the Zaprionus species and broaden our understanding of the diversity of retrotransposons in the Zaprionus genus. (AU)

FAPESP's process: 04/02851-9 - Transposable elements and the impact in plant and bacterial genomes
Grantee:Marie-Anne Van Sluys
Support Opportunities: Research Projects - Thematic Grants