| Full text | |
| Author(s): |
Castilho de Almeida, Natalia Ellen
;
Esteves, Franciele Grego
;
Aparecido dos Santos-Pinto, Jose Roberto
;
de Paula, Carla Peres
;
da Cunha, Anderson Ferreira
;
Malavazi, Iran
;
Palma, Mario Sergio
;
Rodrigues-Filho, Edson
Total Authors: 8
|
| Document type: | Journal article |
| Source: | Journal of Agricultural and Food Chemistry; v. 68, n. 15, p. 8-pg., 2020-04-15. |
| Abstract | |
Celiac disease (CD) is a chronic illness characterized by an inflammatory process triggered by gluten protein intake. Recent evidence has suggested that the lower relative abundance of bifidobacteria in the intestinal lumen may be associated with CD. Herein, we assessed the effect of the Bifidobacterium species Bifidobacterium bifidum, Bifidobacterium longum, Bembidion breve, Bifidobacterium animalis alone, and also a Bifidobacterium consortium on the digestion of intact gluten proteins (gliadins and glutenins) and the associated immunomodulatory responses elicited by the resulting peptides. The cytotoxicity and proinflammatory responses were evaluated through the activation of NF-kappa B p65 and the expression of cytokines TNF-alpha and IL-1 beta in Caco-2 cell cultures exposed to gluten-derived peptides. The peptides induced a clear reduction in cytotoxic responses and proinflammatory marker levels compared to the gluten fragments generated during noninoculated gastrointestinal digestion. These results highlight the possible use of probiotics based on bifidobacteria as a prospective treatment for CD. (AU) | |
| FAPESP's process: | 16/16212-5 - Natural proteopeptides from the Brazilian fauna, flora and microbiota as potential models for the rational development of new drugs of therapeutic use: isolation, structure elucidation, chemical synthesis and functional activity assays |
| Grantee: | Mario Sergio Palma |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 17/19694-3 - The role of heat shock proteins HsfA and Hsp90 and the regulator SmiA at cell wall integrity pathway activity in the opportunistic human pathogen Aspergillus fumigatus |
| Grantee: | Iran Malavazi |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 15/26454-3 - Metabolome of human microbiota associated with the degradation of gluten: an approach involving bifidobacteria |
| Grantee: | Natália Ellen Castilho de Almeida |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 17/10373-0 - Profiling the peptidomic and structural-functional characterization of lipid vesicles present in the Nephila clavipes web spider |
| Grantee: | Franciele Grego Esteves |
| Support Opportunities: | Scholarships in Brazil - Doctorate |