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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of microsatellites markers associated with yield components and quality parameters in sugarcane

Author(s):
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Pinto, L. R. [1] ; Leite, D. C. [1] ; Favero, T. M. [1] ; Pastina, M. M. [2] ; Garcia, A. A. F. [2] ; Perecin, D. ; Goncalves, B. S. [1] ; Creste, S. [1] ; Xavier, M. A. [1] ; Bidoia, M. A. P. [1] ; Landell, M. G. A. [1] ; de Souza, A. Pereira [3]
Total Authors: 12
Affiliation:
[1] Ctr Avangado Pesquisa Tecnol Agronegacio Cana IAC, BR-14001970 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Escola Super Agr Luiz Queiroz ESALQ, Dept Genet, BR-13400970 Piracicaba, SP - Brazil
[3] Univ Estadual Campinas UNICAMP, Ctr Biol Mol Engn Genet CBMEG, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: INTERNATIONAL SUGAR JOURNAL; v. 113, n. 1346, p. 140-144, FEB 2011.
Web of Science Citations: 0
Abstract

Marker assisted selection depends on the identification of tightly linked association between marker and the trait of interest. In the present work, functional (EST-SSRs) and genomic (gSSRs) microsatellite markers were used to detect putative QTLs for sugarcane yield components (stalk number, diameter and height) and as well as for quality parameters (Brix, Pol and fibre) in plant cane. The mapping population (200 individuals) was derived from a bi-parental cross (IACSP95-3018 x IACSP93-3046) from the IAC Sugarcane Breeding Program. As the map is under construction, single marker trait association analysis based on the likelihood ratio test was undertaken to detect the QTLs. Of the 215 single dose markers evaluated (1:1 and 3:1), 90 (42%) were associated with putative QTLs involving 43 microsatellite primers (18 gSSRs and 25 EST-SSRs). For the yield components, 41 marker/trait associations were found: 20 for height, 6 for diameter and 15 for stalk number. An EST-SSRs marker with homology to non-phototropic hypocotyls 4 (NPH4) protein was associated with a putative QTL with positive effect for diameter as also with a negative effect for stalk number. In relation to the quality parameters, 18 marker trait associations were found for Brix, 19 for Pol, and 12 for fibre. For fibre, 58% of the QTLs detected showed a negative effect on this trait. Some makers associated with QTLs with a negative effect for fibre showed a positive effect for Pol, reflecting the negative correlation generally observed between these traits. (AU)

FAPESP's process: 05/55258-6 - Genetic mapping and identification of genetic and functional molecular markers associated with agronomic characteristics of interest in sugarcane
Grantee:Luciana Rossini Pinto Machado da Silva
Support Opportunities: Research Grants - Young Investigators Grants