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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development of microsatellite markers in Guineagrass (Panicum maximum Jacq.) and their transferability to other tropical forage grass species

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Author(s):
Sousa, A. C. B. [1] ; Jungmann, L. [2] ; Campos, T. [1] ; Sforca, D. A. [1] ; Boaventura, L. R. [1] ; Silva, G. M. B. [1] ; Zucchi, M. I. ; Jank, L. [2] ; Souza, A. P. [3, 1]
Total Authors: 9
Affiliation:
[1] Univ Campinas UNICAMP, Ctr Mol Biol & Genet Engn, BR-1308397 Campinas, SP - Brazil
[2] EMBRAPA Beef Cattle, Plant Biotechnol Lab, BR-7900297 Campo Grande, MS - Brazil
[3] Univ Campinas UNICAMP, Plant Biol Dept DBV, Inst Biol, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PLANT BREEDING; v. 130, n. 1, p. 104-108, FEB 2011.
Web of Science Citations: 4
Abstract

P>The Guineagrass (Panicum maximum Jacq.) is one of the most important tropical forage grasses, but genetic knowledge and tools regarding this species are still limited. Therefore, 20 novel polymorphic microsatellite markers were developed, validated, and employed in estimating genetic relationships among 25 P. maximum genotypes selected from a Brazilian germplasm collection. In addition, they were tested for cross-species amplification in four other forage grass species. The number of alleles observed for each locus ranged from 4 to 12 (average 6.7). The values of polymorphism information content (PIC) varied from 0.41 to 0.83 (average 0.61) and the discriminating power (D) ranged from 0.53 to 0.98 (average 0.72). Cross-amplification demonstrated the potential transferability of these microsatellites to four tropical forage grass species. Cluster analysis based on the unweighted pair group method revealed three distinct groups: two clusters consisted of P. maximum genotypes and a third cluster, consisted of the other tropical forage grass species. The data demonstrated that the microsatellites developed herein have potential for germplasm characterization and genetic diversity analysis in P. maximum and other forage grass species. (AU)