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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

APM/CD13 and FOS in the hypothalamus of monosodium glutamate obese and food deprived rats

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Author(s):
Alponti, Rafaela Fadoni [1, 2] ; Nogueira, Maria Ines [2] ; Mendes, Mariana Trivilin [1] ; de Abreu, Cintya [1] ; Silveira, Paulo Flavio [1]
Total Authors: 5
Affiliation:
[1] Inst Butantan, Pharmacol Lab, BR-05503900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Neurosci & Behav, BR-05508030 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Regulatory Peptides; v. 166, n. 1-3, p. 98-104, JAN 17 2011.
Web of Science Citations: 5
Abstract

Protein (western blotting) and gene (PCR) expressions, catalytic activity of puromycin-insensitive membrane-bound neutral aminopeptidase (APM/CD13) and in situ regional distribution of CD13 and FOS immunoreactivity (it) were evaluated in the hypothalamus of monosodium glutamate obese (MSG) and/or food deprived (FD) rats in order to investigate their possible interplay with metabolic functions. Variations in protein and gene expressions of CD13 relative to controls coincided in the hypothalamus of MSG and MSG-FD (decreased 2- to 17-fold). Compared with controls, the reduction of hypothalamic CD13 content reflected a negative balance in its regional distribution in the supraoptic, paraventricular, periventricular and arcuate nuclei. CD13-ir increased in the supraoptic nucleus in MSG (2.5-fold) and decreased in the paraventricular nucleus (2-fold) together with FOS-ir (1.5-fold) in FD. In MSG-FD. FOS-ir decreased (7-fold) in the paraventricular nucleus, while CD13-ir decreased in the periventricular (5.6-fold) and the arcuate (3.7-fold) nuclei. It was noteworthy that all these changes of CD13 were not related to catalytic activity of APM. Data suggested that hypothalamic CD13 plays a role in the regulation of energy metabolism not by means of APM enzyme activity. (c) 2010 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 05/04699-2 - Hypothalamic and hippocampal neutral aminopeptidase (APN/CD13), prolyl dipeptidyl aminopeptidase IV (DPPIV/CD26) and C-FOS in monosodium glutamate-treated and food-deprived rats
Grantee:Paulo Flávio Silveira
Support Opportunities: Regular Research Grants